An optical projection tomography microscope (OPTM) can improve axial resolution by viewing a sample from different perspectives. Here, we report a dual-mode OPTM that can generate 3D images of single cancer cells in both absorption mode and polarization mode. Cancer cells were labeled with hematoxylin for absorption imaging and nanorods for polarization imaging. Absorption images can provide morphologic information, and polarization images can provide molecular information. The combination of molecular detection and 3D cytological cell analysis may help with early cancer diagnosis.
© 2010 Optical Society of America
Original Manuscript: December 2, 2009
Revised Manuscript: February 12, 2010
Manuscript Accepted: February 19, 2010
Published: March 31, 2010
Vol. 5, Iss. 8 Virtual Journal for Biomedical Optics
Qin Miao, Julia Yu, J. Richard Rahn, Michael G. Meyer, Thomas Neumann, Alan C. Nelson, and Eric J. Seibel, "Dual-mode optical projection tomography microscope using gold nanorods and hematoxylin-stained cancer cells," Opt. Lett. 35, 1037-1039 (2010)