Dual-mode optical projection tomography microscope using gold nanorods and hematoxylin-stained cancer cells
Optics Letters, Vol. 35, Issue 7, pp. 1037-1039 (2010)
http://dx.doi.org/10.1364/OL.35.001037
Enhanced HTML 
Acrobat PDF (234 KB)
Abstract
An optical projection tomography microscope (OPTM) can improve axial resolution by viewing a sample from different perspectives. Here, we report a dual-mode OPTM that can generate 3D images of single cancer cells in both absorption mode and polarization mode. Cancer cells were labeled with hematoxylin for absorption imaging and nanorods for polarization imaging. Absorption images can provide morphologic information, and polarization images can provide molecular information. The combination of molecular detection and 3D cytological cell analysis may help with early cancer diagnosis.
© 2010 Optical Society of America
OCIS Codes
(180.6900) Microscopy : Three-dimensional microscopy
(290.5855) Scattering : Scattering, polarization
(110.6955) Imaging systems : Tomographic imaging
ToC Category:
Microscopy
History
Original Manuscript: December 2, 2009
Revised Manuscript: February 12, 2010
Manuscript Accepted: February 19, 2010
Published: March 31, 2010
Virtual Issues
Vol. 5, Iss. 8 Virtual Journal for Biomedical Optics
Citation
Qin Miao, Julia Yu, J. Richard Rahn, Michael G. Meyer, Thomas Neumann, Alan C. Nelson, and Eric J. Seibel, "Dual-mode optical projection tomography microscope using gold nanorods and hematoxylin-stained cancer cells," Opt. Lett. 35, 1037-1039 (2010)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=ol-35-7-1037
You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.
Contact your librarian or system administrator
or
Log in to access OSA Member Subscription
You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.
Contact your librarian or system administrator
or
Log in to access OSA Member Subscription
You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.
Contact your librarian or system administrator
or
Log in to access OSA Member Subscription
You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.
Contact your librarian or system administrator
or
Log in to access OSA Member Subscription
Related Journal Articles 
- Resolution improvement in optical projection tomography by the focal scanning method (OL)
- Optical deflection tomography with the phase-shifting schlieren (OL)
- Single-shot, dual-wavelength digital holography based on polarizing separation (AO)
- Laboratory soft-x-ray microscope for cryotomography of biological specimens (OL)
- 3D imaging of biofilms on implants by detection of scattered light with a scanning laser optical tomograph (BOE)
Related Conference Papers
- GPC-Based Optical Manipulation
- Extended-Resolution Reconstruction of Structured Illumination Microscopy Data
- Dual Modal Three-Dimensional Imaging of Single Cell Using Optical Projection Tomography Microscope
- Nanometric resolution using far-field optical tomographic microscopy in the multiple scattering regime
- Multiplexed Agile Fourier Sampling for Doppler Encoded Excitation Pattern (DEEP) 3D Microscopy
« Previous Article | Next Article »
OSA is a member of 