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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editor: Gregory W. Faris
  • Vol. 5, Iss. 9 — Jul. 6, 2010

Coherent aperture-synthesis, wide-field, high-resolution holographic microscopy of biological tissue

Thomas Gutzler, Timothy R. Hillman, Sergey A. Alexandrov, and David D. Sampson  »View Author Affiliations


Optics Letters, Vol. 35, Issue 8, pp. 1136-1138 (2010)
http://dx.doi.org/10.1364/OL.35.001136


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Abstract

We show for the first time, to our knowledge, high-resolution wide-field images of biological samples recorded using coherent aperture-synthesis Fourier holography. To achieve this, we combined off-axis plane-wave polarized illumination with an axial sample rotation and polarization-sensitive collection of backscattered light. We synthesized 180 Fourier holograms using an efficient postdetection phase-matching correlation scheme. The result was an annular spatial frequency-space synthetic aperture ( NA = 0.93 ) with an effective area 25 times larger than that due to a single hologram. A high-resolution high-contrast microscopic reconstruction of biological tissue was computed over a sample area of 9 mm 2 from holograms acquired at 34 mm working distance.

© 2010 Optical Society of America

OCIS Codes
(000.1430) General : Biology and medicine
(180.3170) Microscopy : Interference microscopy
(290.5850) Scattering : Scattering, particles
(090.1995) Holography : Digital holography

ToC Category:
Holography

History
Original Manuscript: December 22, 2009
Revised Manuscript: February 24, 2010
Manuscript Accepted: February 28, 2010
Published: April 5, 2010

Virtual Issues
Vol. 5, Iss. 9 Virtual Journal for Biomedical Optics

Citation
Thomas Gutzler, Timothy R. Hillman, Sergey A. Alexandrov, and David D. Sampson, "Coherent aperture-synthesis, wide-field, high-resolution holographic microscopy of biological tissue," Opt. Lett. 35, 1136-1138 (2010)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=ol-35-8-1136

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Supplementary Material


» Media 1: MOV (3991 KB)     

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