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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editor: Gregory W. Faris
  • Vol. 5, Iss. 9 — Jul. 6, 2010

Scattered light fluorescence microscopy: imaging through turbid layers

Ivo M. Vellekoop and Christof M. Aegerter  »View Author Affiliations


Optics Letters, Vol. 35, Issue 8, pp. 1245-1247 (2010)
http://dx.doi.org/10.1364/OL.35.001245


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Abstract

A major limitation of any type of microscope is the penetration depth in turbid tissue. Here, we demonstrate a fundamentally novel kind of fluorescence microscope that images through optically thick turbid layers. The microscope uses scattered light, rather than light propagating along a straight path, for imaging with subwavelength resolution. Our method uses constructive interference to focus scattered laser light through the turbid layer. Microscopic fluorescent structures behind the layer were imaged by raster scanning the focus.

© 2010 Optical Society of America

OCIS Codes
(030.6140) Coherence and statistical optics : Speckle
(110.0180) Imaging systems : Microscopy
(110.7050) Imaging systems : Turbid media
(290.4210) Scattering : Multiple scattering

ToC Category:
Imaging Systems

History
Original Manuscript: January 4, 2010
Revised Manuscript: March 11, 2010
Manuscript Accepted: March 12, 2010
Published: April 15, 2010

Virtual Issues
Vol. 5, Iss. 9 Virtual Journal for Biomedical Optics

Citation
Ivo M. Vellekoop and Christof M. Aegerter, "Scattered light fluorescence microscopy: imaging through turbid layers," Opt. Lett. 35, 1245-1247 (2010)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=ol-35-8-1245

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