Investigation of portable in situ fluorescence optical detection for microfluidic 3D cell culture assays
Optics Letters, Vol. 35, Issue 9, pp. 1374-1376 (2010)
http://dx.doi.org/10.1364/OL.35.001374
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Abstract
A portable fluorescence optical detection system was developed to demonstrate real-time in situ analysis of cells that are three-dimensionally cultured in an extracellular matrix under microfluidic environment. The system was designed to provide a large field of view in the lateral plane to average out cellular processes in an axial layer and simultaneously diffraction-limited axial resolution. In this proof-of-concept study, the detection system was applied to quantitative analyses of short-term measurements of cell staining and cell cytotoxicity and long-term monitoring of a cell-invasion assay. For assays, colon cancer cells were cultured in a Matrigel or alginate matrix. The measured data were largely consistent with predicted results and revealed quantitatively cell dynamics specific to 3D cell cultures. The detection system has a potential as a single package to investigate 3D cultures in a microfluidic system.
© 2010 Optical Society of America
OCIS Codes
(120.3890) Instrumentation, measurement, and metrology : Medical optics instrumentation
(170.1530) Medical optics and biotechnology : Cell analysis
(280.4788) Remote sensing and sensors : Optical sensing and sensors
ToC Category:
Instrumentation, Measurement, and Metrology
History
Original Manuscript: January 11, 2010
Revised Manuscript: March 9, 2010
Manuscript Accepted: March 15, 2010
Published: April 23, 2010
Virtual Issues
Vol. 5, Iss. 9 Virtual Journal for Biomedical Optics
Citation
Jong-ryul Choi, Jong Hwan Sung, Michael Louis Shuler, and Donghyun Kim, "Investigation of portable in situ fluorescence optical detection for microfluidic 3D cell culture assays," Opt. Lett. 35, 1374-1376 (2010)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=ol-35-9-1374
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