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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editor: Gregory W. Faris
  • Vol. 5, Iss. 9 — Jul. 6, 2010

Miniaturized selective plane illumination microscopy for high-contrast in vivo fluorescence imaging

Christoph J. Engelbrecht, Fabian Voigt, and Fritjof Helmchen  »View Author Affiliations


Optics Letters, Vol. 35, Issue 9, pp. 1413-1415 (2010)
http://dx.doi.org/10.1364/OL.35.001413


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Abstract

Light-sheet-based fluorescence imaging techniques rely on simultaneous excitation of a single optical plane and thus permit high-contrast optically sectioned imaging of extended tissue samples. Here, we introduce a miniaturized fiber-optic implementation of a selective plane-illumination microscope (miniSPIM). The excitation light was delivered through a single-mode optical fiber, and a light-sheet was created with a cylindrical gradient-index lens and a right-angle microprism. Fluorescence emission was collected orthogonally to the light-sheet through a gradient-index lens assembly and a coherent fiber bundle. The end face of the fiber bundle was imaged onto a charge-coupled device camera. The spatial resolutions of the miniSPIM were 3.2 μ m laterally and 5.1 μ m axially. Images of fluorescent beads and neurons in mouse neocortex exhibited superior axial resolution and contrast in the miniSPIM-mode compared to images recorded in epi-illumination mode. The miniSPIM may thus enable novel in vivo imaging approaches.

© 2010 Optical Society of America

OCIS Codes
(170.0180) Medical optics and biotechnology : Microscopy
(170.2520) Medical optics and biotechnology : Fluorescence microscopy
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(180.2520) Microscopy : Fluorescence microscopy
(110.2945) Imaging systems : Illumination design

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: December 3, 2009
Revised Manuscript: February 17, 2010
Manuscript Accepted: March 8, 2010
Published: April 28, 2010

Virtual Issues
Vol. 5, Iss. 9 Virtual Journal for Biomedical Optics

Citation
Christoph J. Engelbrecht, Fabian Voigt, and Fritjof Helmchen, "Miniaturized selective plane illumination microscopy for high-contrast in vivo fluorescence imaging," Opt. Lett. 35, 1413-1415 (2010)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=ol-35-9-1413


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