OSA's Digital Library

Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics


  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 6, Iss. 7 — Jul. 27, 2011

Focal plane tuning in wide-field-of-view microscope with Talbot pattern illumination

Jigang Wu, Guoan Zheng, Zheng Li, and Changhuei Yang  »View Author Affiliations

Optics Letters, Vol. 36, Issue 12, pp. 2179-2181 (2011)

View Full Text Article

Enhanced HTML    Acrobat PDF (313 KB)

Browse Journals / Lookup Meetings

Browse by Journal and Year


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools



We have developed a focal plane tuning technique for use in focus-grid-based wide-field-of-view microscopy (WFM). In WFM, the incidence of a collimated beam on a mask with a two-dimensional grid of aperture produced the Talbot images of the aperture grid. The Talbot pattern functioned as a focus grid and was used to illuminate the sample. By scanning the sample across the focus grid and collecting the transmission, we can generate a microscopy image of the sample. By tuning the wavelength of the laser, we can tune the focal plane of the WFM and acquire images of different depth into the sample. Images of a green algae microscope slide were acquired at different focal planes for demonstration.

© 2011 Optical Society of America

OCIS Codes
(050.1970) Diffraction and gratings : Diffractive optics
(170.0110) Medical optics and biotechnology : Imaging systems
(170.5810) Medical optics and biotechnology : Scanning microscopy

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: April 15, 2011
Manuscript Accepted: May 1, 2011
Published: June 6, 2011

Virtual Issues
Vol. 6, Iss. 7 Virtual Journal for Biomedical Optics

Jigang Wu, Guoan Zheng, Zheng Li, and Changhuei Yang, "Focal plane tuning in wide-field-of-view microscope with Talbot pattern illumination," Opt. Lett. 36, 2179-2181 (2011)

Sort:  Author  |  Year  |  Journal  |  Reset  


  1. J. Ho, A. V. Parwani, D. M. Jukic, Y. Yagi, L. Anthony, and J. R. Gilbertson, Hum. Pathol. 37, 322 (2006). [CrossRef] [PubMed]
  2. M. Oheim, Br. J. Pharmacol. 152, 1 (2007). [CrossRef] [PubMed]
  3. W. Bishara, T. W. Su, A. F. Coskun, and A. Ozcan, Opt. Express 18, 11181 (2010). [CrossRef] [PubMed]
  4. M. G. Rojo, G. B. Garcia, C. P. Mateos, J. G. Garcia, and M. C. Vicente, Int. J. Surg. Pathol. 14, 285 (2006). [CrossRef] [PubMed]
  5. J. Wu, X. Cui, G. Zheng, Y. M. Wang, L. M. Lee, and C. Yang, Opt. Lett. 35, 2188 (2010). [CrossRef] [PubMed]
  6. R. Graf, J. Rietdorf, and T. Zimmermann, Adv. Biochem. Eng. Biotechnol. 95, 1311 (2005).
  7. J. R. Leger and G. J. Swanson, Opt. Lett. 15, 288 (1990). [CrossRef] [PubMed]
  8. H. F. Talbot, Philos. Mag. 9, 401 (1836).
  9. K. Patorski, Prog. Opt. 27, 1 (1989). [CrossRef]
  10. B. Besold and N. Lindlein, Opt. Eng. 36, 1099 (1997). [CrossRef]
  11. E. di Mambro, R. Haidar, N. Guerineau, and J. Primot, J. Opt. Soc. Am. A 21, 2276 (2004). [CrossRef]

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.


Fig. 1 Fig. 2 Fig. 3

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited