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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 6, Iss. 7 — Jul. 27, 2011

Contrast enhancement in widefield CARS microscopy by tailored phase matching using a spatial light modulator

Alexander Jesacher, Clemens Roider, Saranjam Khan, Gregor Thalhammer, Stefan Bernet, and Monika Ritsch-Marte  »View Author Affiliations


Optics Letters, Vol. 36, Issue 12, pp. 2245-2247 (2011)
http://dx.doi.org/10.1364/OL.36.002245


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Abstract

We introduce a widefield CARS microscope implementation that uses a spatial light modulator to obtain extremely precise control over the pump/probe-beam incidence geometry, which provides the possibility to enhance the image contrast at specific target resonances by fine-tuning the incidence angles. We show how this technique can be used to optimize the image contrast between objects of different size and to practically eliminate the undesired signal from the solvent that embeds small target specimens. Changing the numerical aperture of the illumination from 1.27 to 1.24 improved the ratio of the signals of 500 nm polystyrene beads and the agarose solvent by about 20 dB .

© 2011 Optical Society of America

OCIS Codes
(180.0180) Microscopy : Microscopy
(190.5650) Nonlinear optics : Raman effect
(290.5910) Scattering : Scattering, stimulated Raman

ToC Category:
Microscopy

History
Original Manuscript: April 1, 2011
Revised Manuscript: May 2, 2011
Manuscript Accepted: May 8, 2011
Published: June 8, 2011

Virtual Issues
Vol. 6, Iss. 7 Virtual Journal for Biomedical Optics

Citation
Alexander Jesacher, Clemens Roider, Saranjam Khan, Gregor Thalhammer, Stefan Bernet, and Monika Ritsch-Marte, "Contrast enhancement in widefield CARS microscopy by tailored phase matching using a spatial light modulator," Opt. Lett. 36, 2245-2247 (2011)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=ol-36-12-2245


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