|
Contrast enhancement in widefield CARS microscopy by tailored phase matching using a spatial light modulator |
Optics Letters, Vol. 36, Issue 12, pp. 2245-2247 (2011)
http://dx.doi.org/10.1364/OL.36.002245
Enhanced HTML 
Acrobat PDF (456 KB)
Abstract
We introduce a widefield CARS microscope implementation that uses a spatial light modulator to obtain extremely precise control over the pump/probe-beam incidence geometry, which provides the possibility to enhance the image contrast at specific target resonances by fine-tuning the incidence angles. We show how this technique can be used to optimize the image contrast between objects of different size and to practically eliminate the undesired signal from the solvent that embeds small target specimens. Changing the numerical aperture of the illumination from 1.27 to 1.24 improved the ratio of the signals of
© 2011 Optical Society of America
OCIS Codes
(180.0180) Microscopy : Microscopy
(190.5650) Nonlinear optics : Raman effect
(290.5910) Scattering : Scattering, stimulated Raman
ToC Category:
Microscopy
History
Original Manuscript: April 1, 2011
Revised Manuscript: May 2, 2011
Manuscript Accepted: May 8, 2011
Published: June 8, 2011
Virtual Issues
Vol. 6, Iss. 7 Virtual Journal for Biomedical Optics
Citation
Alexander Jesacher, Clemens Roider, Saranjam Khan, Gregor Thalhammer, Stefan Bernet, and Monika Ritsch-Marte, "Contrast enhancement in widefield CARS microscopy by tailored phase matching using a spatial light modulator," Opt. Lett. 36, 2245-2247 (2011)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=ol-36-12-2245
Sort: Year | Journal | Reset
References
- M. D. Duncan, J. Reintjes, and T. J. Manuccia, Opt. Lett. 7, 350 (1982). [CrossRef] [PubMed]
- A. Zumbusch, G. R. Holtom, and X. S. Xie, Phys. Rev. Lett. 82, 4142 (1999). [CrossRef]
- M. Müller and A. Zumbusch, Chem. Phys. Chem. 8, 2156(2007). [CrossRef] [PubMed]
- C. Heinrich, S. Bernet, and M. Ritsch-Marte, Appl. Phys. Lett. 84, 816 (2004). [CrossRef]
- I. Toytman, K. Cohn, T. Smith, D. Simanovskii, and D. Palanker, Opt. Lett. 32, 1941 (2007). [CrossRef] [PubMed]
- C. Heinrich, S. Bernet, and M. Ritsch-Marte, New J. Phys. 8, 36 (2006). [CrossRef]
- I. Toytman, D. Simanovskii, and D. Palanker, Opt. Express 17, 7339 (2009). [CrossRef] [PubMed]
- A. Volkmer, J. X. Cheng, and X. S. Xie, Phys. Rev. Lett. 87, 023901 (2001). [CrossRef]
- M. Müller, J. Squier, C. A. De Lange, and G. J. Brakenhoff, J. Microsc. 197, 150 (2000). [CrossRef] [PubMed]
- R. Fiolka, M. Beck, and A. Stemmer, Opt. Lett. 33, 1629(2008). [CrossRef] [PubMed]
Cited By |
 Alert me when this paper is cited |
OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.
Multimedia
| Multimedia Files | Recommended Software |
| » Media 1: AVI (1126 KB) | QuickTime |
Related Journal Articles 
- Raman gain measurement in solid parahydrogen (OL)
- Characterization of a continuous-wave Raman laser in H2 (JOSAB)
- Frequency tuning characteristics of a continuous-wave Raman laser in H2 (JOSAB)
- Consideration of Stimulated Raman Scattering in Yb:Sr5(PO4)3F Laser Amplifiers (AO)
- Observation of Raman conversion for 70-fs pulses in KGd(WO 4) 2 crystal in the regime of impulsive stimulated Raman scattering (OL)
Related Conference Papers
- Broad bandwidth, stimulated Raman scattering in optical fibers.
- CARS Microscopy: Biomedical Imaging by Nonlinear Vibrational Spectroscopy
- Raman Amplification of Low Divergent Radiation in Barium Nitrate
- Chemically-Specific Photoacoustic Imaging using Vibrational Raman Excitation
- Chemically-Specific Photoacoustic Imaging using Vibrational Raman Excitation
- Chemically-Specific Photoacoustic Imaging using Vibrational Raman Excitation
- Chemically-Specific Photoacoustic Imaging using Vibrational Raman Excitation
« Previous Article | Next Article »
OSA is a member of 