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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 6, Iss. 8 — Aug. 26, 2011

Time-resolved detection enables standard two-photon fluorescence microscopy for in vivo label-free imaging of microvasculature in tissue

Dong Li, Wei Zheng, Wei Zhang, Seng Khoon Teh, Yan Zeng, Yi Luo, and Jianan Y. Qu  »View Author Affiliations


Optics Letters, Vol. 36, Issue 14, pp. 2638-2640 (2011)
http://dx.doi.org/10.1364/OL.36.002638


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Abstract

We conducted a systematic study on two-photon excited fluorescence (TPEF) of hemoglobin using the near transform-limited and Gaussian-shaped femtosecond pulse sources. We found that the two-photon action cross section of hemoglobin drops over 2 orders of magnitude in the wavelength range from 550 to 800 nm , while the spectral and temporal characteristics of hemoglobin TPEF are insensitive to the change of excitation wavelength. In particular, our new findings showed that the hemoglobin fluorescence could be excited with sufficient efficiency using a conventional Ti:sapphire laser tuned at the wavelength close to 700 nm . With the employment of a time-resolved detection method, we demonstrated that the TPEF signals of hemoglobin excited by a Ti:sapphire laser could be clearly differentiated from other nonlinear signals presented within the living biological tissues, indicating that a standard TPEF microscope can become a routine tool for in vivo label-free microangiography imaging.

© 2011 Optical Society of America

OCIS Codes
(170.1470) Medical optics and biotechnology : Blood or tissue constituent monitoring
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.6920) Medical optics and biotechnology : Time-resolved imaging
(180.6900) Microscopy : Three-dimensional microscopy
(180.4315) Microscopy : Nonlinear microscopy

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: April 4, 2011
Revised Manuscript: June 9, 2011
Manuscript Accepted: June 9, 2011
Published: July 12, 2011

Virtual Issues
Vol. 6, Iss. 8 Virtual Journal for Biomedical Optics

Citation
Dong Li, Wei Zheng, Wei Zhang, Seng Khoon Teh, Yan Zeng, Yi Luo, and Jianan Y. Qu, "Time-resolved detection enables standard two-photon fluorescence microscopy for in vivo label-free imaging of microvasculature in tissue," Opt. Lett. 36, 2638-2640 (2011)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=ol-36-14-2638

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Supplementary Material


» Media 1: AVI (2357 KB)     
» Media 2: AVI (2310 KB)     

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