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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 6, Iss. 8 — Aug. 26, 2011

Laboratory soft-x-ray microscope for cryotomography of biological specimens

Michael Bertilson, Olov von Hofsten, Ulrich Vogt, Anders Holmberg, Athanasia E. Christakou, and Hans M. Hertz  »View Author Affiliations


Optics Letters, Vol. 36, Issue 14, pp. 2728-2730 (2011)
http://dx.doi.org/10.1364/OL.36.002728


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Abstract

Soft-x-ray cryotomography allows quantitative and high-resolution three-dimensional imaging of intact unstained cells. To date, the method relies on synchrotron-radiation sources, which limits accessibility for re searchers. Here we present a laboratory water-window microscope for cryotomography. It is based on a λ = 2 . 48 nm liquid-jet laser-plasma source, a normal-incidence multilayer condenser, a 30 nm zone-plate objective, and a cryotilt sample holder. We demonstrate high-resolution imaging, as well as quantitative tomographic imaging, of frozen intact cells. The reconstructed tomogram of the intracellular local absorption coefficient shows details down to 100 nm .

© 2011 Optical Society of America

OCIS Codes
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(180.6900) Microscopy : Three-dimensional microscopy
(180.7460) Microscopy : X-ray microscopy
(340.0340) X-ray optics : X-ray optics
(110.6955) Imaging systems : Tomographic imaging

ToC Category:
Microscopy

History
Original Manuscript: April 15, 2011
Manuscript Accepted: May 12, 2011
Published: July 14, 2011

Virtual Issues
Vol. 6, Iss. 8 Virtual Journal for Biomedical Optics

Citation
Michael Bertilson, Olov von Hofsten, Ulrich Vogt, Anders Holmberg, Athanasia E. Christakou, and Hans M. Hertz, "Laboratory soft-x-ray microscope for cryotomography of biological specimens," Opt. Lett. 36, 2728-2730 (2011)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=ol-36-14-2728

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Supplementary Material


» Media 1: MOV (3809 KB)     
» Media 2: MOV (2632 KB)     

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