OSA's Digital Library

Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 6, Iss. 9 — Oct. 3, 2011

Dielectric microsphere mediated transfection using a femtosecond laser

Mitsuhiro Terakawa and Yuto Tanaka  »View Author Affiliations


Optics Letters, Vol. 36, Issue 15, pp. 2877-2879 (2011)
http://dx.doi.org/10.1364/OL.36.002877


View Full Text Article

Enhanced HTML    Acrobat PDF (401 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations

Abstract

We demonstrate the permeabilization of cell membranes by an enhanced optical field generated under polystyrene microspheres of 1000 nm diameter excited by a femtosecond laser pulse. Fluorescent molecules and short interfering RNA (siRNA) have been successfully delivered to many cells in the irradiated area by a single 80 fs laser pulse at 800 nm wavelength in the presence of antibody-conjugated polystyrene spheres. The ratios of the cells showing permeabilization were 38% and 21% for Fluorescein isothiocyanate-dextran and siRNA, respectively, at the laser fluence of 1.06 J / cm 2 . The present method has advantages both in high throughput of many cell treatments and precise processing of minute areas on cell membranes.

© 2011 Optical Society of America

OCIS Codes
(140.3390) Lasers and laser optics : Laser materials processing
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology
(290.4020) Scattering : Mie theory

ToC Category:
Lasers and Laser Optics

History
Original Manuscript: May 12, 2011
Manuscript Accepted: June 27, 2011
Published: July 25, 2011

Virtual Issues
Vol. 6, Iss. 9 Virtual Journal for Biomedical Optics

Citation
Mitsuhiro Terakawa and Yuto Tanaka, "Dielectric microsphere mediated transfection using a femtosecond laser," Opt. Lett. 36, 2877-2879 (2011)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=ol-36-15-2877


Sort:  Author  |  Year  |  Journal  |  Reset  

References

  1. M. Tsukakoshi, S. Kurata, Y. Nomiya, Y. Ikawa, and T. Kasuya, Appl. Phys. B 35, 135 (1984). [CrossRef]
  2. Y. Arita, M. L. Torres-Mapa, W. M. Lee, T. Čižmár, P. Campbell, F. J. Gunn-Moore, and K. Dholakia, Appl. Phys. Lett. 98, 093702 (2011). [CrossRef]
  3. M. L. Torres-Mapa, L. Angus, M. Ploschner, K. Dholakia, and F. J. Gunn-Moore, J. Biomed. Opt. 15, 041506 (2010). [CrossRef] [PubMed]
  4. U. K. Tirlapur and K. König, Nature 418, 290 (2002). [CrossRef] [PubMed]
  5. A. Vogel, J. Noack, G. Hüttman, and G. Paltauf, Appl. Phys. B 81, 1015 (2005). [CrossRef]
  6. S. Kumar, I. Z. Maxwell, A. Heisterkamp, T. R. Polte, T. P. Lele, M. Salanga, E. Mazur, and D. E. Ingber, Biophys. J. 90, 3762 (2006). [CrossRef] [PubMed]
  7. P. Mthunzi, K. Dholakia, and F. Gunn-Moore, J. Biomed. Opt. 15, 041507 (2010). [CrossRef] [PubMed]
  8. A. G. Doukas and N. Kollias, Adv. Drug Delivery Rev. 56, 559 (2004). [CrossRef]
  9. M. Terakawa, M. Ogura, S. Sato, H. Wakisaka, H. Ashida, M. Uenoyama, Y. Masaki, and M. Obara, Opt. Lett. 29, 1227 (2004). [CrossRef] [PubMed]
  10. W. R. Lee, S. C. Shen, C. R. Liu, C. L. Fang, C. H. Hu, and J. Y. Fang, J. Control. Release 115, 344 (2006). [CrossRef] [PubMed]
  11. T. H. Wu, S. Kalim, C. Callahan, M. Teitell, and P. Y. Chiou, Opt. Express 18, 938 (2010). [CrossRef] [PubMed]
  12. X. Huang, P. K. Jain, I. H. El-Sayed, and M. A. El-Sayed, Lasers Med. Sci. 23, 217 (2008). [CrossRef]
  13. C. M. Pitsillides, E. K. Joe, X. Wei, R. R. Anderson, and C. P. Lin, Biophys. J. 84, 4023 (2003). [CrossRef] [PubMed]
  14. C. Yao, X. Qu, Z. Zhang, G. Hüttman, and R. Rahmanzadeh, J. Biomed. Opt. 14, 054034 (2009). [CrossRef] [PubMed]
  15. J. Baumgart, L. Humbert, B. S. Lalonde, J. J. Lebrun, and M. Meunier, Proc. SPIE 7925, 79250I (2011). [CrossRef]
  16. M. Schomaker, J. Baumgart, D. Motekaitis, D. Heinemann, J. Kwawinkel, M. Pangalos, W. Bintig, E. Boulais, R. Lachaine, B. S. Lalonde, A. Ngezahayo, M. Meunier, and A. Heisterkamp, Proc. SPIE 7925, 79250F (2011). [CrossRef]
  17. Y. Pan, S. Neuss, A. Leifert, M. Fischler, F. Wen, U. Simon, G. Schmid, W. Brandau, and W. Jahnen-Dechent, Small 3, 1941 (2007). [CrossRef] [PubMed]
  18. Y. Chen, Y. Hung, I. Liau, and G. S. Huang, Nanoscale Res. Lett. 4, 858 (2009). [CrossRef] [PubMed]
  19. Z. B. Wang, J. Joseph, L. Li, and B. S. Luk’yanchuk, Proc. Inst. Mech. Eng. C 224, 1113 (2010). [CrossRef]
  20. T. Sakai, Y. Tanaka, Y. Nishizawa, M. Terakawa, and M. Obara, Appl. Phys. A 99, 39 (2010). [CrossRef]
  21. Y. Tanaka, G. Obara, A. Zenidaka, M. Terakawa, and M. Obara, Appl. Phys. Lett. 96, 201103 (2010). [CrossRef]
  22. G. Olivié, D. Giguère, F. Vidal, T. Ozaki, J.-C. Kieffer, O. Nada, and I. Brunette, Opt. Express 16, 4121 (2008). [CrossRef] [PubMed]
  23. M. F. Yanik, H. Cinar, H. N. Cinar, A. D. Chisholm, Y. Jin, and A. Ben-Yakar, Nature 432, 822 (2004). [CrossRef] [PubMed]
  24. A. Tien, S. Backus, H. Kapteyn, M. Murnane, and G. Mourou, Phys. Rev. Lett. 82, 3883 (1999). [CrossRef]

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.

Figures

Fig. 1 Fig. 2 Fig. 3
 

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited