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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 6, Iss. 9 — Oct. 3, 2011

Driving and analysis of micro-objects by digital holographic microscope in microfluidics

F. Merola, L. Miccio, M. Paturzo, A. Finizio, S. Grilli, and P. Ferraro  »View Author Affiliations


Optics Letters, Vol. 36, Issue 16, pp. 3079-3081 (2011)
http://dx.doi.org/10.1364/OL.36.003079


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Abstract

We propose an optical configuration in which floating particles in a microfluidic chamber can be characterized by an interference microscopy configuration to obtain quantitative phase-contrast maps. The configuration is simply made by two laser beams from the same laser source. One beam provides the optical forces for driving the particle along appropriate paths, but at same time works as the object illumination beam in the holographic microscope. The second beam plays the role of the reference beam, allowing recording of an interference fringe pattern (i.e., the digital hologram) in an out-of-focus image plane. The system and method are illustrated and experimental results are offered for polymeric particles as well as for in vitro cells with the aim to demonstrate the approach.

© 2011 Optical Society of America

OCIS Codes
(090.1995) Holography : Digital holography
(350.4855) Other areas of optics : Optical tweezers or optical manipulation

ToC Category:
Holography

History
Original Manuscript: May 19, 2011
Revised Manuscript: July 8, 2011
Manuscript Accepted: July 11, 2011
Published: August 8, 2011

Virtual Issues
Vol. 6, Iss. 9 Virtual Journal for Biomedical Optics

Citation
F. Merola, L. Miccio, M. Paturzo, A. Finizio, S. Grilli, and P. Ferraro, "Driving and analysis of micro-objects by digital holographic microscope in microfluidics," Opt. Lett. 36, 3079-3081 (2011)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=ol-36-16-3079

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Supplementary Material


» Media 1: MOV (3699 KB)     
» Media 2: MOV (944 KB)     

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