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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 6, Iss. 2 — Feb. 17, 2011

High-speed synthetic aperture microscopy for live cell imaging

Moonseok Kim, Youngwoon Choi, Christopher Fang-Yen, Yongjin Sung, Ramachandra R. Dasari, Michael S. Feld, and Wonshik Choi  »View Author Affiliations


Optics Letters, Vol. 36, Issue 2, pp. 148-150 (2011)
http://dx.doi.org/10.1364/OL.36.000148


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Abstract

We present a high-speed synthetic aperture microscopy for quantitative phase imaging of live biological cells. We measure 361 complex amplitude images of an object with various directions of illumination covering an NA of 0.8 in less than one-thirteenth of a second and then combine the images with a phase-referencing method to create a synthesized phase image. Because of the increased depth selectivity, artifacts from diffraction that are typically present in coherent imaging are significantly suppressed, and lateral resolution of phase imaging is improved. We use the instrument to demonstrate high-quality phase imaging of live cells, both static and dynamic, and thickness measurements of a nanoscale cholesterol helical ribbon.

© 2011 Optical Society of America

OCIS Codes
(100.6950) Image processing : Tomographic image processing
(110.1650) Imaging systems : Coherence imaging
(100.3175) Image processing : Interferometric imaging
(110.3175) Imaging systems : Interferometric imaging

ToC Category:
Image Processing

History
Original Manuscript: October 22, 2010
Revised Manuscript: November 30, 2010
Manuscript Accepted: December 3, 2010
Published: January 6, 2011

Virtual Issues
Vol. 6, Iss. 2 Virtual Journal for Biomedical Optics

Citation
Moonseok Kim, Youngwoon Choi, Christopher Fang-Yen, Yongjin Sung, Ramachandra R. Dasari, Michael S. Feld, and Wonshik Choi, "High-speed synthetic aperture microscopy for live cell imaging," Opt. Lett. 36, 148-150 (2011)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=ol-36-2-148


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