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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics


  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 7, Iss. 1 — Jan. 4, 2012

Phase-shifting Zernike phase contrast microscopy for quantitative phase measurement

Peng Gao, Baoli Yao, Irina Harder, Norbert Lindlein, and Francisco Jose Torcal-Milla  »View Author Affiliations

Optics Letters, Vol. 36, Issue 21, pp. 4305-4307 (2011)

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Zernike phase contrast microscopy is extended and combined with a phase-shifting mechanism to perform quantitative phase measurements of microscopic objects. Dozens of discrete point light sources on a ring are constructed for illumination. For each point light source, three different levels of point-like phase steps are designed, which are alternatively located along a ring on a silica plate to perform phase retardation on the undiffracted (dc) component of the object waves. These three levels of the phase steps are respectively selected by rotating the silica plate. Thus, quantitative evaluation of phase specimens can be performed via phase-shifting mechanism. The proposed method has low “halo” and “shade-off” effects, low coherent noise level, and high lateral resolution due to the improved illumination scheme.

© 2011 Optical Society of America

OCIS Codes
(070.6110) Fourier optics and signal processing : Spatial filtering
(100.5090) Image processing : Phase-only filters
(170.0180) Medical optics and biotechnology : Microscopy
(180.0180) Microscopy : Microscopy

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: July 14, 2011
Revised Manuscript: September 18, 2011
Manuscript Accepted: September 20, 2011
Published: November 1, 2011

Virtual Issues
Vol. 7, Iss. 1 Virtual Journal for Biomedical Optics

Peng Gao, Baoli Yao, Irina Harder, Norbert Lindlein, and Francisco Jose Torcal-Milla, "Phase-shifting Zernike phase contrast microscopy for quantitative phase measurement," Opt. Lett. 36, 4305-4307 (2011)

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