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Fast Fourier domain localization algorithm of a single molecule with nanometer precision |
Optics Letters, Vol. 36, Issue 22, pp. 4317-4319 (2011)
http://dx.doi.org/10.1364/OL.36.004317
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Abstract
We present an algorithm to determine the location of a fluorescent molecule with nanometer-scale accuracy. A Fourier domain localization scheme based on zero-padded fast Fourier transform and phase gradient operators is used to obtain a powerful mathematical model for localizing the molecule without numerical fitting. Compared with conventional algorithms, our position estimator does not require prior background information or initial parameter estimation. Numerical simulations indicate that the proposed method exhibits high localization precision and small bias while executing almost as fast as the fluoroBancroft algorithm.
© 2011 Optical Society of America
OCIS Codes
(100.2960) Image processing : Image analysis
(100.6640) Image processing : Superresolution
(180.2520) Microscopy : Fluorescence microscopy
ToC Category:
Image Processing
History
Original Manuscript: June 22, 2011
Revised Manuscript: August 20, 2011
Manuscript Accepted: September 29, 2011
Published: November 9, 2011
Virtual Issues
Vol. 7, Iss. 1 Virtual Journal for Biomedical Optics
Citation
Bin Yu, Danni Chen, Junle Qu, and Hanben Niu, "Fast Fourier domain localization algorithm of a single molecule with nanometer precision," Opt. Lett. 36, 4317-4319 (2011)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=ol-36-22-4317
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