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Optical fiber-based photomechanical gene transfer system for in vivo application |
Optics Letters, Vol. 36, Issue 23, pp. 4545-4547 (2011)
http://dx.doi.org/10.1364/OL.36.004545
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Abstract
We developed an optical-fiber-based photomechanical gene transfer system for endoscopic or catheter-based application. A fiber tip with a laser-absorbing film covered with a transparent plastic disk for plasma confinement was attached to a quartz fiber; the film was irradiated with nanosecond laser pulses transmitted through the fiber to generate photomechanical waves (PMWs). Characteristics of PMWs emitted from the fiber tip were examined to confirm the necessary conditions for gene transfer. We then attempted to transfer reporter genes to the rat skin as a test tissue in vivo with the fiber system, and the results showed significantly high protein levels and spatially selective pinpoint gene expressions in the tissue.
© 2011 Optical Society of America
OCIS Codes
(140.3540) Lasers and laser optics : Lasers, Q-switched
(170.3890) Medical optics and biotechnology : Medical optics instrumentation
ToC Category:
Medical Optics and Biotechnology
History
Original Manuscript: September 8, 2011
Revised Manuscript: October 19, 2011
Manuscript Accepted: October 19, 2011
Published: November 23, 2011
Virtual Issues
Vol. 7, Iss. 2 Virtual Journal for Biomedical Optics
Citation
Shunichi Sato, Takahiro Ando, and Minoru Obara, "Optical fiber-based photomechanical gene transfer system for in vivo application," Opt. Lett. 36, 4545-4547 (2011)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=ol-36-23-4545
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