OSA's Digital Library

Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 6, Iss. 3 — Mar. 18, 2011

Spectral-domain differential interference contrast microscopy

Yizheng Zhu, Natan T. Shaked, Lisa L. Satterwhite, and Adam Wax  »View Author Affiliations


Optics Letters, Vol. 36, Issue 4, pp. 430-432 (2011)
http://dx.doi.org/10.1364/OL.36.000430


View Full Text Article

Enhanced HTML    Acrobat PDF (364 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations

Abstract

We present a fiber-optic low-coherence imaging technique, termed spectral-domain differential interference contrast microscopy (SD-DIC), for quantitative DIC imaging of both reflective surfaces and transparent biological specimens. SD-DIC combines the common-path nature of a Nomarski DIC interferometer with the high sensitivity of spectral-domain low-coherence interferometry to obtain high-resolution, quantitative measurements of optical pathlength gradients from a single point on the sample. Full-field imaging can be achieved by scanning the sample. A reflected-light SD-DIC system was demonstrated using a USAF resolution target as the phase object. Live cardiomyocytes were also imaged, achieving a resolution of 36 pm for pathlength gradient measurements. The dynamics of cardiomyocyte contraction were recorded with high sensitivity at selected sites on the cells.

© 2011 Optical Society of America

OCIS Codes
(110.0180) Imaging systems : Microscopy
(120.3180) Instrumentation, measurement, and metrology : Interferometry
(170.5810) Medical optics and biotechnology : Scanning microscopy
(180.3170) Microscopy : Interference microscopy

ToC Category:
Imaging Systems

History
Original Manuscript: October 6, 2010
Revised Manuscript: December 7, 2010
Manuscript Accepted: December 21, 2010
Published: January 31, 2011

Virtual Issues
Vol. 6, Iss. 3 Virtual Journal for Biomedical Optics

Citation
Yizheng Zhu, Natan T. Shaked, Lisa L. Satterwhite, and Adam Wax, "Spectral-domain differential interference contrast microscopy," Opt. Lett. 36, 430-432 (2011)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=ol-36-4-430

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited