The scanning two-photon fluorescence microscope produces optically sectioned images from the focal plane. It is sometimes desirable to acquire images from other planes of the specimen that are inclined with respect to the focal plane. In this Letter, we discuss the issues concerned with acquiring such images together with the effects of the inclination angle on image resolution and sectioning strength. To obtain images from oblique planes at high speed, a two-photon system was built wherein a novel optical system is used to provide aberration-free scanning.
© 2011 Optical Society of America
Original Manuscript: September 24, 2010
Manuscript Accepted: October 28, 2010
Published: February 23, 2011
Vol. 6, Iss. 4 Virtual Journal for Biomedical Optics
Christopher W. Smith, Edward J. Botcherby, Martin J. Booth, Rimas Juškaitis, and Tony Wilson, "Agitation-free multiphoton microscopy of oblique planes," Opt. Lett. 36, 663-665 (2011)