We investigate the range of application of single-beam homodyne spectral phase interferometry for direct electric field reconstruction (SPIDER) for multiphoton microscopy. Simulations and experimental studies performed on model spectral phase profiles show that the phase reconstruction technique used in this method makes the phase retrieval quality highly sensitive to the complexity of the profile. In addition, we show that the use of iterative processes is likely to deteriorate the phase retrieval quality, especially for strongly varying phase profiles. These effects are illustrated and quantified for sinusoidal and quadratic spectral phase profiles.

© 2011 Optical Society of America

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