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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 6, Iss. 4 — May. 4, 2011

Adaptive optics wide-field microscopy using direct wavefront sensing

Oscar Azucena, Justin Crest, Shaila Kotadia, William Sullivan, Xiaodong Tao, Marc Reinig, Donald Gavel, Scot Olivier, and Joel Kubby  »View Author Affiliations


Optics Letters, Vol. 36, Issue 6, pp. 825-827 (2011)
http://dx.doi.org/10.1364/OL.36.000825


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Abstract

We report a technique for measuring and correcting the wavefront aberrations introduced by a biological sample using a Shack–Hartmann wavefront sensor, a fluorescent reference source, and a deformable mirror. The reference source and sample fluorescence are at different wavelengths to separate wavefront measurement and sample imaging. The measurement and correction at one wavelength improves the resolving power at a different wavelength, enabling the structure of the sample to be resolved.

© 2011 Optical Society of America

OCIS Codes
(010.7350) Atmospheric and oceanic optics : Wave-front sensing
(160.2540) Materials : Fluorescent and luminescent materials
(220.1080) Optical design and fabrication : Active or adaptive optics

ToC Category:
Optical Design and Fabrication

History
Original Manuscript: November 22, 2010
Revised Manuscript: January 31, 2011
Manuscript Accepted: February 1, 2011
Published: March 8, 2011

Virtual Issues
Vol. 6, Iss. 4 Virtual Journal for Biomedical Optics

Citation
Oscar Azucena, Justin Crest, Shaila Kotadia, William Sullivan, Xiaodong Tao, Marc Reinig, Donald Gavel, Scot Olivier, and Joel Kubby, "Adaptive optics wide-field microscopy using direct wavefront sensing," Opt. Lett. 36, 825-827 (2011)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=ol-36-6-825

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