OSA's Digital Library

Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 7, Iss. 3 — Feb. 29, 2012

Fabrication of optical mosaics mimicking human corneal endothelium for the training and assessment of eye bank technicians

Manuel Flury, Zhiguo He, Nelly Campolmi, Philippe Gain, Bernard Kress, and Gilles Thuret  »View Author Affiliations


Optics Letters, Vol. 37, Issue 1, pp. 22-24 (2012)
http://dx.doi.org/10.1364/OL.37.000022


View Full Text Article

Enhanced HTML    Acrobat PDF (546 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations

Abstract

The determination of endothelial cell density (ECD) is a crucial activity in eye banks for the assessment of corneal tissue quality. These cells are responsible for corneal transparency, and ECD correlates with graft survival. ECD is mainly assessed with a manual “naked-eye” procedure under a transmitted light microscope in Europe and using a specular microscope in the United States. Interbank and intrabank variability has been previously demonstrated. In order to facilitate training and continuing education of technicians and reliability assessment of eye banks’ ECD determination, we use micro-optics technologies to fabricate test mosaics that exactly reproduce the image of human corneal endothelium. The description of the fabrication process is detailed, and comparisons are made between amplitude and phase mosaics.

© 2012 Optical Society of America

OCIS Codes
(170.0180) Medical optics and biotechnology : Microscopy
(170.4470) Medical optics and biotechnology : Ophthalmology
(220.4000) Optical design and fabrication : Microstructure fabrication

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: July 20, 2011
Revised Manuscript: November 14, 2011
Manuscript Accepted: November 14, 2011
Published: December 22, 2011

Virtual Issues
Vol. 7, Iss. 3 Virtual Journal for Biomedical Optics

Citation
Manuel Flury, Zhiguo He, Nelly Campolmi, Philippe Gain, Bernard Kress, and Gilles Thuret, "Fabrication of optical mosaics mimicking human corneal endothelium for the training and assessment of eye bank technicians," Opt. Lett. 37, 22-24 (2012)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=ol-37-1-22


Sort:  Author  |  Year  |  Journal  |  Reset  

References

  1. J. K. Nishimura, D. O. Hodge, and W. M. Bourne, Ophthalmology 106, 1962 (1999). [CrossRef]
  2. S. V. Patel, D. O. Hodge, and W. M. Bourne, Am. J. Ophthalmol. 139, 311 (2005). [CrossRef]
  3. European Eye Bank Association Directory, 19th ed.(European Eye Bank Association, 2011).
  4. Eye Bank Association of America. Medical Standards (European Eye Bank Association, 2010).
  5. E. Pels, H. Beele, and I. Claerhout, Int. Ophthalmol. 28, 155 (2008). [CrossRef]
  6. G. Thuret, C. Manissolle, S. Acquart, J. C. Le Petit, J. Maugery, L. Campos-Guyotat, M. J. Doughty, and P. Gain, Br. J. Ophthalmol. 87, 1481 (2003). [CrossRef]
  7. G. Thuret, C. Manissolle, S. Acquart, O. Garraud, L. Campos-Guyotat, J. Maugery, and P. Gain, Transplantation 78, 1299 (2004). [CrossRef]
  8. N. Deb-Joardar, G. Thuret, G. A. Racine, D. Pons, G. Brun, O. Parriaux, M. Peoc’h, S. Acquart, and P. Gain, Investig. Ophthalmol. Vis. Sci. 47, 4373 (2006). [CrossRef]
  9. B. Kress and P. Meyrueis, Applied Digital Optics: From Micro-Optics to Nanophotonics (Wiley, 2009).

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.

Figures

Fig. 1. Fig. 2. Fig. 3.
 
Fig. 4.
 

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited