OSA's Digital Library

Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 7, Iss. 7 — Jun. 25, 2012

Targeted alteration of real and imaginary refractive index of biological cells by histological staining

L. Cherkezyan, H. Subramanian, V. Stoyneva, J. D. Rogers, S. Yang, D. Damania, A. Taflove, and V. Backman  »View Author Affiliations


Optics Letters, Vol. 37, Issue 10, pp. 1601-1603 (2012)
http://dx.doi.org/10.1364/OL.37.001601


View Full Text Article

Enhanced HTML    Acrobat PDF (409 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations

Abstract

Various staining techniques are commonly used in biomedical research to investigate cellular morphology. By inducing absorption of light, staining dyes change the intracellular refractive index due to the Kramers–Kronig relationship. We present a method for creating 2D maps of real and imaginary refractive indices of stained biological cells using their thickness and absorptance. We validate our technique on dyed polystyrene microspheres and quantify the alteration in refractive index of stained biological cells. We reveal that specific staining of individual organelles can increase their scattering cross-section by orders of magnitudes, implying a major impact in the field of biophotonics.

© 2012 Optical Society of America

OCIS Codes
(160.4760) Materials : Optical properties
(170.0180) Medical optics and biotechnology : Microscopy
(170.1530) Medical optics and biotechnology : Cell analysis
(290.0290) Scattering : Scattering
(300.6550) Spectroscopy : Spectroscopy, visible

ToC Category:
Materials

History
Original Manuscript: December 2, 2011
Revised Manuscript: January 27, 2012
Manuscript Accepted: February 11, 2012
Published: May 9, 2012

Virtual Issues
Vol. 7, Iss. 7 Virtual Journal for Biomedical Optics

Citation
L. Cherkezyan, H. Subramanian, V. Stoyneva, J. D. Rogers, S. Yang, D. Damania, A. Taflove, and V. Backman, "Targeted alteration of real and imaginary refractive index of biological cells by histological staining," Opt. Lett. 37, 1601-1603 (2012)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=ol-37-10-1601


Sort:  Author  |  Year  |  Journal  |  Reset  

References

  1. E. D. P. De Robertis, F. A. Saez, and E. M. F. De Robertis, Cell Biology, 6th ed. (Saunders, 1975).
  2. E. S. Cibas and B. S. Ducatman, Cytology: Diagnostic Principles and Clinical Correlates (Saunders, 1996).
  3. J. D. Wilson, W. J. Cottrell, and T. H. Foster, J. Biomed. Opt. 12, 014010 (2007). [CrossRef]
  4. R. d. L. Kronig, J. Opt. Soc. Am. 12, 547 (1926). [CrossRef]
  5. H. A. Kramers, Atti del Congresso Internazionale dei Fisici 2, 545 (1927). [CrossRef]
  6. S. Uttam, R. K. Bista, D. J. Hartman, R. E. Brand, and Y. Liu, J. Biomed. Opt. 16, 116013 (2011). [CrossRef]
  7. V. Lucarini, Kramers-Kronig Relations in Optical Materials Research (Springer, 2005).
  8. H. C. v. d. Hulst, Light Scattering by Small Particles (Wiley, 1957).
  9. S. A. Prahl, “Inverse adding-doubling software,” http://omlc.ogi.edu/software/iad/index.html .
  10. J. W. Pickering, S. A. Prahl, N. Vanwieringen, J. F. Beek, H. J. C. M. Sterenborg, and M. J. C. Vangemert, Appl. Opt. 32, 399 (1993). [CrossRef]
  11. S. A. Prahl, M. J. C. Vangemert, and A. J. Welch, Appl. Opt. 32, 559 (1993). [CrossRef]
  12. C. Maetzler, “MATLAB functions for Mie scattering and absorption,” Research Report 2002-08 (Bern, 2002).
  13. D. J. Cook, in Cellular Pathology, 2nd ed. (Scion, 2006), pp. 67–104.
  14. G. C. Crossmon, Stain Technol. 24, 241 (1949). [CrossRef]
  15. H. G. Davies, M. H. F. Wilkins, J. Chayen, and L. F. Lacour, Q. J. Microsc. Sci. 95, 271 (1954).
  16. J. M. Schmitt and G. Kumar, Appl. Opt. 37, 2788 (1998). [CrossRef]
  17. G. W. Kattawar and G. N. Plass, Appl. Opt. 6, 1377(1967). [CrossRef]
  18. N. N. Boustany, S. A. Boppart, and V. Backman, Annu. Rev. Biomed. Eng. 12, 285 (2010). [CrossRef]
  19. H. Subramanian, P. Pradhan, Y. Liu, I. R. Capoglu, J. D. Rogers, H. K. Roy, R. E. Brand, and V. Backman, Opt. Lett. 34, 518 (2009). [CrossRef]

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.

Figures

Fig. 1. Fig. 2. Fig. 3.
 

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited