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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 7, Iss. 8 — Aug. 2, 2012

Quantitative pupil analysis in stimulated emission depletion microscopy using phase retrieval

Emil B. Kromann, Travis J. Gould, Manuel F. Juette, Jens E. Wilhjelm, and Joerg Bewersdorf  »View Author Affiliations


Optics Letters, Vol. 37, Issue 11, pp. 1805-1807 (2012)
http://dx.doi.org/10.1364/OL.37.001805


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Abstract

The resolution attainable with stimulated emission depletion (STED) microscopy greatly depends on the quality of the STED laser focus. So far, visual inspection of a measured STED focus has been the only convenient means of gauging the source of aberrations. Here we describe a method, requiring no instrument modifications, for obtaining an equivalent to the complex pupil function at the back aperture of the objective and show that it provides quantitative information about aberration sources (including aberrations induced by the objective or sample). We show the accuracy of this field representation to be sufficient for reconstructing the STED focus in three dimensions and determining corrective steps.

© 2012 Optical Society of America

OCIS Codes
(100.5070) Image processing : Phase retrieval
(100.6640) Image processing : Superresolution
(170.5810) Medical optics and biotechnology : Scanning microscopy
(080.4865) Geometric optics : Optical vortices

ToC Category:
Image Processing

History
Original Manuscript: February 14, 2012
Manuscript Accepted: February 22, 2012
Published: May 17, 2012

Virtual Issues
Vol. 7, Iss. 8 Virtual Journal for Biomedical Optics

Citation
Emil B. Kromann, Travis J. Gould, Manuel F. Juette, Jens E. Wilhjelm, and Joerg Bewersdorf, "Quantitative pupil analysis in stimulated emission depletion microscopy using phase retrieval," Opt. Lett. 37, 1805-1807 (2012)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=ol-37-11-1805


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