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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics


  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 7, Iss. 8 — Aug. 2, 2012

Pupil-segmentation-based adaptive optical correction of a high-numerical-aperture gradient refractive index lens for two-photon fluorescence endoscopy

Chen Wang and Na Ji  »View Author Affiliations

Optics Letters, Vol. 37, Issue 11, pp. 2001-2003 (2012)

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The intrinsic aberrations of high-NA gradient refractive index (GRIN) lenses limit their image quality as well as field of view. Here we used a pupil-segmentation-based adaptive optical approach to correct the inherent aberrations in a two-photon fluorescence endoscope utilizing a 0.8 NA GRIN lens. By correcting the field-dependent aberrations, we recovered diffraction-limited performance across a large imaging field. The consequent improvements in imaging signal and resolution allowed us to detect fine structures that were otherwise invisible inside mouse brain slices.

© 2012 Optical Society of America

OCIS Codes
(110.0180) Imaging systems : Microscopy
(110.1080) Imaging systems : Active or adaptive optics

ToC Category:
Imaging Systems

Original Manuscript: March 9, 2012
Manuscript Accepted: April 10, 2012
Published: May 30, 2012

Virtual Issues
Vol. 7, Iss. 8 Virtual Journal for Biomedical Optics

Chen Wang and Na Ji, "Pupil-segmentation-based adaptive optical correction of a high-numerical-aperture gradient refractive index lens for two-photon fluorescence endoscopy," Opt. Lett. 37, 2001-2003 (2012)

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  1. C. Gomez-Reino, M. V. Perez, and C. Bao, Gradient-Index Optics (Springer-Verlag, 2002).
  2. W. J. Tomlinson, Appl. Opt. 19, 1127 (1980). [CrossRef]
  3. J. C. Jung and M. J. Schnitzer, Opt. Lett. 28, 902 (2003). [CrossRef]
  4. W. M. Lee and S. H. Yun, Opt. Lett. 36, 4608 (2011). [CrossRef]
  5. F. Bortoletto, C. Bonoli, P. Panizzolo, C. D. Ciubotaru, and F. Mammano, PLoS ONE 6, e22321 (2011). [CrossRef]
  6. N. Ji, D. E. Milkie, and E. Betzig, Nat. Meth. 7, 141 (2010). [CrossRef]
  7. N. Ji, T. R. Sato, and E. Betzig, Proc. Nat. Acad. Sci. USA 109, 22 (2012). [CrossRef]
  8. R. P. Barretto, B. Messerschmidt, and M. J. Schnitzer, Nat. Meth. 6, 511 (2009). [CrossRef]
  9. GRINTECH. Datasheet “High NA for 2-Photon Microscopy,” available from http://www.grintech.de/grin-lens-systems-for-medical-applications.html .

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