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Pupil-segmentation-based adaptive optical correction of a high-numerical-aperture gradient refractive index lens for two-photon fluorescence endoscopy |
Optics Letters, Vol. 37, Issue 11, pp. 2001-2003 (2012)
http://dx.doi.org/10.1364/OL.37.002001
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Abstract
The intrinsic aberrations of high-NA gradient refractive index (GRIN) lenses limit their image quality as well as field of view. Here we used a pupil-segmentation-based adaptive optical approach to correct the inherent aberrations in a two-photon fluorescence endoscope utilizing a 0.8 NA GRIN lens. By correcting the field-dependent aberrations, we recovered diffraction-limited performance across a large imaging field. The consequent improvements in imaging signal and resolution allowed us to detect fine structures that were otherwise invisible inside mouse brain slices.
© 2012 Optical Society of America
OCIS Codes
(110.0180) Imaging systems : Microscopy
(110.1080) Imaging systems : Active or adaptive optics
ToC Category:
Imaging Systems
History
Original Manuscript: March 9, 2012
Manuscript Accepted: April 10, 2012
Published: May 30, 2012
Virtual Issues
Vol. 7, Iss. 8 Virtual Journal for Biomedical Optics
Citation
Chen Wang and Na Ji, "Pupil-segmentation-based adaptive optical correction of a high-numerical-aperture gradient refractive index lens for two-photon fluorescence endoscopy," Opt. Lett. 37, 2001-2003 (2012)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=ol-37-11-2001
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References
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