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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 7, Iss. 8 — Aug. 2, 2012

Pupil-segmentation-based adaptive optical correction of a high-numerical-aperture gradient refractive index lens for two-photon fluorescence endoscopy

Chen Wang and Na Ji  »View Author Affiliations


Optics Letters, Vol. 37, Issue 11, pp. 2001-2003 (2012)
http://dx.doi.org/10.1364/OL.37.002001


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Abstract

The intrinsic aberrations of high-NA gradient refractive index (GRIN) lenses limit their image quality as well as field of view. Here we used a pupil-segmentation-based adaptive optical approach to correct the inherent aberrations in a two-photon fluorescence endoscope utilizing a 0.8 NA GRIN lens. By correcting the field-dependent aberrations, we recovered diffraction-limited performance across a large imaging field. The consequent improvements in imaging signal and resolution allowed us to detect fine structures that were otherwise invisible inside mouse brain slices.

© 2012 Optical Society of America

OCIS Codes
(110.0180) Imaging systems : Microscopy
(110.1080) Imaging systems : Active or adaptive optics

ToC Category:
Imaging Systems

History
Original Manuscript: March 9, 2012
Manuscript Accepted: April 10, 2012
Published: May 30, 2012

Virtual Issues
Vol. 7, Iss. 8 Virtual Journal for Biomedical Optics

Citation
Chen Wang and Na Ji, "Pupil-segmentation-based adaptive optical correction of a high-numerical-aperture gradient refractive index lens for two-photon fluorescence endoscopy," Opt. Lett. 37, 2001-2003 (2012)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=ol-37-11-2001


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References

  1. C. Gomez-Reino, M. V. Perez, and C. Bao, Gradient-Index Optics (Springer-Verlag, 2002).
  2. W. J. Tomlinson, Appl. Opt. 19, 1127 (1980). [CrossRef]
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  8. R. P. Barretto, B. Messerschmidt, and M. J. Schnitzer, Nat. Meth. 6, 511 (2009). [CrossRef]
  9. GRINTECH. Datasheet “High NA for 2-Photon Microscopy,” available from http://www.grintech.de/grin-lens-systems-for-medical-applications.html .

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