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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics


  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 7, Iss. 8 — Aug. 2, 2012

Label-free morphometry of retinal nerve fiber bundles by second-harmonic-generation microscopy

Hyungsik Lim and John Danias  »View Author Affiliations

Optics Letters, Vol. 37, Issue 12, pp. 2316-2318 (2012)

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We present an application of second-harmonic generation (SHG) microscopy for label-free visualization and quantification of the morphology of nerve fibers in the retina. We show that SHG arises from the retinal nerve fiber layer and that it is specifically associated with uniformly oriented microtubules in the axons. The utility of axonal SHG is demonstrated for imaging the neuroanatomy of fresh ex vivo retina, and the three-dimensional structure of the axons of retinal ganglion cells is quantitatively analyzed.

© 2012 Optical Society of America

OCIS Codes
(170.4470) Medical optics and biotechnology : Ophthalmology
(180.6900) Microscopy : Three-dimensional microscopy
(180.4315) Microscopy : Nonlinear microscopy

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: March 22, 2012
Manuscript Accepted: April 13, 2012
Published: June 8, 2012

Virtual Issues
Vol. 7, Iss. 8 Virtual Journal for Biomedical Optics

Hyungsik Lim and John Danias, "Label-free morphometry of retinal nerve fiber bundles by second-harmonic-generation microscopy," Opt. Lett. 37, 2316-2318 (2012)

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