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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 7, Iss. 8 — Aug. 2, 2012

Multi-color miniature dual-axis confocal microscope for point-of-care pathology

Steven Y. Leigh and Jonathan T. C. Liu  »View Author Affiliations


Optics Letters, Vol. 37, Issue 12, pp. 2430-2432 (2012)
http://dx.doi.org/10.1364/OL.37.002430


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Abstract

We present a miniature microelectromechanical systems–based dual-axis confocal microscope capable of spatially coregistered fluorescence and reflectance imaging at multiple wavelengths. This device has a 10 mm diameter scan head with a 2 mm diameter tip for convenient use during surgery to guide tumor resection. The microscope has an adjustable focal depth of 20–200 micrometers and is capable of imaging with an axial resolution of 9 micrometers and in-plane resolution of 4 micrometers over a field of view of 450×450 micrometers. Simultaneous two-color imaging of individual optical sections is achieved by using a pair of grating–prism assemblies to compensate for chromatic dispersion in the 2 mm diameter gradient index relay lens at the distal tip of the device. Experimental measurements of the axial response of the microscope, as well as two-color images of a reflective bar target and fresh mouse brain tissues, demonstrate the performance of our device and its potential for multicolor in vivo optical sectioning microscopy.

© 2012 Optical Society of America

OCIS Codes
(050.1970) Diffraction and gratings : Diffractive optics
(170.0180) Medical optics and biotechnology : Microscopy
(170.1790) Medical optics and biotechnology : Confocal microscopy
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.5810) Medical optics and biotechnology : Scanning microscopy
(220.1000) Optical design and fabrication : Aberration compensation

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: April 9, 2012
Manuscript Accepted: April 24, 2012
Published: June 15, 2012

Virtual Issues
Vol. 7, Iss. 8 Virtual Journal for Biomedical Optics

Citation
Steven Y. Leigh and Jonathan T. C. Liu, "Multi-color miniature dual-axis confocal microscope for point-of-care pathology," Opt. Lett. 37, 2430-2432 (2012)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=ol-37-12-2430


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