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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics


  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 7, Iss. 9 — Aug. 28, 2012

Fluorescence holography with improved signal-to-noise ratio by near image plane recording

Xiaomin Lai, Yuan Zhao, Xiaohua Lv, Zhenqiao Zhou, and Shaoqun Zeng  »View Author Affiliations

Optics Letters, Vol. 37, Issue 13, pp. 2445-2447 (2012)

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Holographic fluorescence imaging is very promising, as it can obtain three-dimensional fluorescence imaging without scanning. However, the current method usually records holograms far from the image plane, with the fluorescence decaying when spreading broadly. Here we show that the signal-to-noise ratio (SNR) of fluorescence holography can be improved by recording the high-contrast interferogram near the image plane. We found that this can be achieved by setting the focal length of the lens for the reference wave (f2) close to that for the object wave (f1). With experiments, we demonstrate an example of an increase of about 21 times in SNR by changing f2 from infinity to 226 mm, which is close to f1 (323 mm).

© 2012 Optical Society of America

OCIS Codes
(090.2880) Holography : Holographic interferometry
(110.6880) Imaging systems : Three-dimensional image acquisition
(170.2520) Medical optics and biotechnology : Fluorescence microscopy
(090.1995) Holography : Digital holography

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Original Manuscript: March 12, 2012
Revised Manuscript: April 9, 2012
Manuscript Accepted: April 19, 2012
Published: June 18, 2012

Virtual Issues
Vol. 7, Iss. 9 Virtual Journal for Biomedical Optics

Xiaomin Lai, Yuan Zhao, Xiaohua Lv, Zhenqiao Zhou, and Shaoqun Zeng, "Fluorescence holography with improved signal-to-noise ratio by near image plane recording," Opt. Lett. 37, 2445-2447 (2012)

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