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Fast and precise algorithm based on maximum radial symmetry for single molecule localization |
Optics Letters, Vol. 37, Issue 13, pp. 2481-2483 (2012)
http://dx.doi.org/10.1364/OL.37.002481
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Abstract
We present an algorithm to estimate the location of single fluorescent molecule with both high speed and high precision. This algorithm is based on finding the subpixel position with maximum radial symmetry in a pixelated single molecule fluorescence image. Compared with conventional algorithms, this algorithm does not rely on point-spread-function or noise model. Through numerical simulation and experimental analysis, we found that this algorithm exhibits localization precision very close to the maximum likelihood estimator (MLE), while executes
© 2012 Optical Society of America
OCIS Codes
(100.6640) Image processing : Superresolution
(110.2960) Imaging systems : Image analysis
(180.2520) Microscopy : Fluorescence microscopy
ToC Category:
Microscopy
History
Original Manuscript: March 2, 2012
Revised Manuscript: March 26, 2012
Manuscript Accepted: April 25, 2012
Published: June 19, 2012
Virtual Issues
Vol. 7, Iss. 9 Virtual Journal for Biomedical Optics
Citation
Hongqiang Ma, Fan Long, Shaoqun Zeng, and Zhen-Li Huang, "Fast and precise algorithm based on maximum radial symmetry for single molecule localization," Opt. Lett. 37, 2481-2483 (2012)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=ol-37-13-2481
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