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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 7, Iss. 9 — Aug. 28, 2012

Label-free in vivo flow cytometry in zebrafish using two-photon autofluorescence imaging

Yan Zeng, Jin Xu, Dong Li, Li Li, Zilong Wen, and Jianan Y. Qu  »View Author Affiliations


Optics Letters, Vol. 37, Issue 13, pp. 2490-2492 (2012)
http://dx.doi.org/10.1364/OL.37.002490


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Abstract

We demonstrate a label-free in vivo flow cytometry in zebrafish blood vessels based on two-photon excited autofluorescence imaging. The major discovery in this work is the strong autofluorescence emission from the plasma in zebrafish blood. The plasma autofluorescence provides excellent contrast for visualizing blood vessels and counting blood cells. In addition, the cellular nicotinamide adenine dinucleotide autofluorescence enables in vivo imaging and counting of white blood cells (neutrophils).

© 2012 Optical Society of America

OCIS Codes
(170.1470) Medical optics and biotechnology : Blood or tissue constituent monitoring
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(180.5810) Microscopy : Scanning microscopy
(180.4315) Microscopy : Nonlinear microscopy
(170.6935) Medical optics and biotechnology : Tissue characterization

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: February 13, 2012
Revised Manuscript: April 9, 2012
Manuscript Accepted: May 7, 2012
Published: June 20, 2012

Virtual Issues
Vol. 7, Iss. 9 Virtual Journal for Biomedical Optics

Citation
Yan Zeng, Jin Xu, Dong Li, Li Li, Zilong Wen, and Jianan Y. Qu, "Label-free in vivo flow cytometry in zebrafish using two-photon autofluorescence imaging," Opt. Lett. 37, 2490-2492 (2012)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=ol-37-13-2490

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