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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 7, Iss. 9 — Aug. 28, 2012

Ultrafast, large-field multiphoton microscopy based on an acousto-optic deflector and a spatial light modulator

Yonghong Shao, Wan Qin, Honghai Liu, Junle Qu, Xiang Peng, Hanben Niu, and Bruce Z. Gao  »View Author Affiliations


Optics Letters, Vol. 37, Issue 13, pp. 2532-2534 (2012)
http://dx.doi.org/10.1364/OL.37.002532


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Abstract

We present an ultrafast, large-field multiphoton excitation fluorescence microscope with high lateral and axial resolutions based on a two-dimensional (2-D) acousto-optical deflector (AOD) scanner and spatial light modulator (SLM). When a phase-only SLM is used to shape the near-infrared light from a mode-locked titanium:sapphire laser into a multifocus array including the 0-order beam, a 136μm×136μm field of view is achieved with a 60× objective using a 2-D AOD scanner without any mechanical scan element. The two-photon fluorescence image of a neuronal network that was obtained using this system demonstrates that our microscopy permits observation of dynamic biological events in a large field with high-temporal and -spatial resolution.

© 2012 Optical Society of America

OCIS Codes
(110.0180) Imaging systems : Microscopy
(170.7160) Medical optics and biotechnology : Ultrafast technology
(180.2520) Microscopy : Fluorescence microscopy
(180.6900) Microscopy : Three-dimensional microscopy

ToC Category:
Microscopy

History
Original Manuscript: March 6, 2012
Revised Manuscript: April 29, 2012
Manuscript Accepted: May 9, 2012
Published: June 21, 2012

Virtual Issues
Vol. 7, Iss. 9 Virtual Journal for Biomedical Optics

Citation
Yonghong Shao, Wan Qin, Honghai Liu, Junle Qu, Xiang Peng, Hanben Niu, and Bruce Z. Gao, "Ultrafast, large-field multiphoton microscopy based on an acousto-optic deflector and a spatial light modulator," Opt. Lett. 37, 2532-2534 (2012)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=ol-37-13-2532

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