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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 7, Iss. 9 — Aug. 28, 2012

Line-scanning detection instrument for photonic crystal enhanced fluorescence

Vikram Chaudhery, Meng Lu, Cheng-Sheng Huang, James Polans, Ruimin Tan, Richard C. Zangar, and Brian T. Cunningham  »View Author Affiliations


Optics Letters, Vol. 37, Issue 13, pp. 2565-2567 (2012)
http://dx.doi.org/10.1364/OL.37.002565


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Abstract

A laser line-scanning instrument was developed to optimize the near-field enhancement capability of a one-dimensional photonic crystal (PC) for excitation of surface-bound fluorophores. The excitation laser beam is shaped into an 8μm×1mm line that is focused along the direction of the PC grating, while remaining collimated perpendicular to the grating. Such a beam configuration offers high excitation power density while simultaneously providing high resonant coupling efficiency from the laser to the PC surface. Using a panel of 21 immunofluorescence assays on the PC surface in a microarray format, the approach achieves an enhancement factor as high as 90-fold between on-resonance and off-resonance illumination. The instrument provides a capability for sensitive and inexpensive analysis of cancer biomarkers in clinical applications.

© 2012 Optical Society of America

OCIS Codes
(180.2520) Microscopy : Fluorescence microscopy
(130.5296) Integrated optics : Photonic crystal waveguides
(050.5298) Diffraction and gratings : Photonic crystals

ToC Category:
Diffraction and Gratings

History
Original Manuscript: February 16, 2012
Revised Manuscript: May 4, 2012
Manuscript Accepted: May 6, 2012
Published: June 21, 2012

Virtual Issues
Vol. 7, Iss. 9 Virtual Journal for Biomedical Optics

Citation
Vikram Chaudhery, Meng Lu, Cheng-Sheng Huang, James Polans, Ruimin Tan, Richard C. Zangar, and Brian T. Cunningham, "Line-scanning detection instrument for photonic crystal enhanced fluorescence," Opt. Lett. 37, 2565-2567 (2012)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=ol-37-13-2565

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