Mitochondrial oxygen tension can be measured in vivo by means of oxygen-dependent quenching of delayed fluorescence of protoporphyrin IX (PpIX). Here we demonstrate that mitochondrial ${\mathrm{PO}}_2$ ($\text{mito}{\mathrm{PO}}_2$) can be measured in the skin of a rat after topical application of the PpIX precursor 5-aminolevulinic acid (ALA). Calibration of $\text{mito}{\mathrm{PO}}_2$ measurements was done by comparison with simultaneous measurements of the cutaneous microvascular ${\mathrm{PO}}_2$ This was done under three different conditions: in normal skin tissue, in nonrespiration skin tissue due to the application of cyanide, and in anoxic skin tissue after the ventilation with 100% nitrogen. The results of this study show that it is feasible to measure the $\text{mito}{\mathrm{PO}}_2$ after the topical application of ALA cream by means of the PpIX–triplet state lifetime technique.

© 2012 Optical Society of America

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