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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 7, Iss. 9 — Aug. 28, 2012

Tomographic imaging of a suspending single live cell using optical tweezer-combined full-field optical coherence tomography

Woo June Choi, Kwan Seob Park, Tae Joong Eom, Myung-Kyu Oh, and Byeong Ha Lee  »View Author Affiliations


Optics Letters, Vol. 37, Issue 14, pp. 2784-2786 (2012)
http://dx.doi.org/10.1364/OL.37.002784


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Abstract

We propose a label-free depth-resolved tomographic scheme for imaging a single live cell in fluid. This approach utilizes a modified time-domain full-field optical coherence tomography (FF-OCT) system combined with an optical tweezer technique. The optical trap for holding a moving specimen is made by tightly focusing a 1064 nm Q-switching pulsed laser beam with a 1.0 NA microscope objective in the sample arm of the FF-OCT part. By cosharing the probe for both systems, the optical actions of trapping and cellular resolution tomographic imaging could be achieved simultaneously. Feasibility of the combined system is demonstrated by imaging micron-sized polystyrene beads and a living suspension cell in medium.

© 2012 Optical Society of America

OCIS Codes
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.4500) Medical optics and biotechnology : Optical coherence tomography
(170.6900) Medical optics and biotechnology : Three-dimensional microscopy
(350.4855) Other areas of optics : Optical tweezers or optical manipulation

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: April 4, 2012
Revised Manuscript: May 1, 2012
Manuscript Accepted: May 10, 2012
Published: July 3, 2012

Virtual Issues
Vol. 7, Iss. 9 Virtual Journal for Biomedical Optics

Citation
Woo June Choi, Kwan Seob Park, Tae Joong Eom, Myung-Kyu Oh, and Byeong Ha Lee, "Tomographic imaging of a suspending single live cell using optical tweezer-combined full-field optical coherence tomography," Opt. Lett. 37, 2784-2786 (2012)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=ol-37-14-2784


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