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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics


  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 7, Iss. 10 — Oct. 5, 2012

Spectroscopic diffraction phase microscopy

Hoa Pham, Basanta Bhaduri, Huafeng Ding, and Gabriel Popescu  »View Author Affiliations

Optics Letters, Vol. 37, Issue 16, pp. 3438-3440 (2012)

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We present spectroscopic diffraction phase microscopy (sDPM) as a method capable of measuring quantitative phase images at multiple wavelengths. sDPM uses a spatial light modulator at the Fourier plane of a lens to select desired wavelengths from the white light illumination of a grating. The quantitative phase information at different wavelengths allows us to decouple the refractive index and the thickness from the phase shift induced by biological cells. We demonstrate the capability of the setup by dispersion measurements of microsphere beads and RBCs.

© 2012 Optical Society of America

OCIS Codes
(120.5050) Instrumentation, measurement, and metrology : Phase measurement
(170.1470) Medical optics and biotechnology : Blood or tissue constituent monitoring
(170.6510) Medical optics and biotechnology : Spectroscopy, tissue diagnostics
(180.3170) Microscopy : Interference microscopy

ToC Category:

Original Manuscript: May 14, 2012
Manuscript Accepted: July 13, 2012
Published: August 10, 2012

Virtual Issues
Vol. 7, Iss. 10 Virtual Journal for Biomedical Optics

Hoa Pham, Basanta Bhaduri, Huafeng Ding, and Gabriel Popescu, "Spectroscopic diffraction phase microscopy," Opt. Lett. 37, 3438-3440 (2012)

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