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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics


  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 7, Iss. 11 — Oct. 31, 2012

Single-exposure complementary aperture phase microscopy with polarization encoding

Kengyeh K. Chu and Jerome Mertz  »View Author Affiliations

Optics Letters, Vol. 37, Issue 18, pp. 3798-3800 (2012)

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We introduce a single-exposure widefield system of producing phase gradient images by dividing the illumination and detection apertures into two oblique complementary components and encoding them using polarization. A Wollaston prism splits the images formed by the two respective aperture halves to allow both components to be simultaneously imaged by a single camera. By producing images characteristically similar to differential interference contrast while using a darkfield illumination scheme, sensitivity to weak phase gradients is improved.

© 2012 Optical Society of America

OCIS Codes
(110.0180) Imaging systems : Microscopy
(110.1220) Imaging systems : Apertures
(110.4980) Imaging systems : Partial coherence in imaging

ToC Category:
Imaging Systems

Original Manuscript: May 30, 2012
Revised Manuscript: July 20, 2012
Manuscript Accepted: August 8, 2012
Published: September 6, 2012

Virtual Issues
Vol. 7, Iss. 11 Virtual Journal for Biomedical Optics

Kengyeh K. Chu and Jerome Mertz, "Single-exposure complementary aperture phase microscopy with polarization encoding," Opt. Lett. 37, 3798-3800 (2012)

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