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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics


  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 7, Iss. 12 — Dec. 19, 2012

High average brightness water window source for short-exposure cryomicroscopy

D. H. Martz, M. Selin, O. von Hofsten, E. Fogelqvist, A. Holmberg, U. Vogt, H. Legall, G. Blobel, C. Seim, H. Stiel, and H. M. Hertz  »View Author Affiliations

Optics Letters, Vol. 37, Issue 21, pp. 4425-4427 (2012)

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Laboratory water window cryomicroscopy has recently demonstrated similar image quality as synchrotron-based microscopy but still with much longer exposure times, prohibiting the spread to a wider scientific community. Here we demonstrate high-resolution laboratory water window imaging of cryofrozen cells with 10 s range exposure times. The major improvement is the operation of a λ=2.48nm, 2 kHz liquid nitrogen jet laser plasma source with high spatial and temporal stability at high average brightness >1.5×1012ph/(s×sr×μm2×line), i.e., close to that of early synchrotrons. Thus, this source enables not only biological x-ray microscopy in the home laboratory but potentially other applications previously only accessible at synchrotron facilities.

© 2012 Optical Society of America

OCIS Codes
(340.7460) X-ray optics : X-ray microscopy
(340.7480) X-ray optics : X-rays, soft x-rays, extreme ultraviolet (EUV)

ToC Category:
X-ray Optics

Original Manuscript: July 13, 2012
Revised Manuscript: September 18, 2012
Manuscript Accepted: September 19, 2012
Published: October 22, 2012

Virtual Issues
Vol. 7, Iss. 12 Virtual Journal for Biomedical Optics

D. H. Martz, M. Selin, O. von Hofsten, E. Fogelqvist, A. Holmberg, U. Vogt, H. Legall, G. Blobel, C. Seim, H. Stiel, and H. M. Hertz, "High average brightness water window source for short-exposure cryomicroscopy," Opt. Lett. 37, 4425-4427 (2012)

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