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Addressable discrete-line-scanning multiphoton microscopy based on a spatial light modulator |
Optics Letters, Vol. 37, Issue 5, pp. 827-829 (2012)
http://dx.doi.org/10.1364/OL.37.000827
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Abstract
We developed a novel addressable discrete-line-scanning multiphoton microscope with high lateral and axial resolutions based on a spatial light modulator. Our discrete-line-focus design eliminates the cross talk that occurs in conventional one-dimensional line-scanning multiphoton microscopies. Additionally, a phase-only spatial light modulator is able to scan only a sample’s target area by generating a specific discrete line focus according to the shape and location of the target area. Compared with other multiphoton microscopies, this technique shortens scanning time and minimizes photodamage by concentrating scanning energy and dwell time on the area of interest.
© 2012 Optical Society of America
OCIS Codes
(170.7160) Medical optics and biotechnology : Ultrafast technology
(180.2520) Microscopy : Fluorescence microscopy
(180.6900) Microscopy : Three-dimensional microscopy
(180.4315) Microscopy : Nonlinear microscopy
ToC Category:
Microscopy
History
Original Manuscript: October 3, 2011
Revised Manuscript: January 5, 2012
Manuscript Accepted: January 5, 2012
Published: February 22, 2012
Virtual Issues
Vol. 7, Iss. 5 Virtual Journal for Biomedical Optics
Citation
Wan Qin, Yonghong Shao, Honghai Liu, Xiang Peng, Hanben Niu, and Bruce Gao, "Addressable discrete-line-scanning multiphoton microscopy based on a spatial light modulator," Opt. Lett. 37, 827-829 (2012)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=ol-37-5-827
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