We report a scheme for the detector system of confocal microscopes in which the pinhole and a large-area detector are substituted by a CCD camera. The numerical integration of the intensities acquired by the active pixels emulates the signal passing through the pinhole. We demonstrate the imaging capability and the optical sectioning of the system. Subtractive-imaging confocal microscopy can be implemented in a simple manner, providing superresolution and improving optical sectioning.
© 2012 Optical Society of America
Original Manuscript: January 12, 2012
Revised Manuscript: February 13, 2012
Manuscript Accepted: February 16, 2012
Published: March 30, 2012
Vol. 7, Iss. 6 Virtual Journal for Biomedical Optics
Emilio Sánchez-Ortiga, Colin J. R. Sheppard, Genaro Saavedra, Manuel Martínez-Corral, Ana Doblas, and Arnau Calatayud, "Subtractive imaging in confocal scanning microscopy using a CCD camera as a detector," Opt. Lett. 37, 1280-1282 (2012)