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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 7, Iss. 6 — May. 25, 2012

Subtractive imaging in confocal scanning microscopy using a CCD camera as a detector

Emilio Sánchez-Ortiga, Colin J. R. Sheppard, Genaro Saavedra, Manuel Martínez-Corral, Ana Doblas, and Arnau Calatayud  »View Author Affiliations


Optics Letters, Vol. 37, Issue 7, pp. 1280-1282 (2012)
http://dx.doi.org/10.1364/OL.37.001280


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Abstract

We report a scheme for the detector system of confocal microscopes in which the pinhole and a large-area detector are substituted by a CCD camera. The numerical integration of the intensities acquired by the active pixels emulates the signal passing through the pinhole. We demonstrate the imaging capability and the optical sectioning of the system. Subtractive-imaging confocal microscopy can be implemented in a simple manner, providing superresolution and improving optical sectioning.

© 2012 Optical Society of America

OCIS Codes
(180.0180) Microscopy : Microscopy
(180.1790) Microscopy : Confocal microscopy
(180.5810) Microscopy : Scanning microscopy

ToC Category:
Microscopy

History
Original Manuscript: January 12, 2012
Revised Manuscript: February 13, 2012
Manuscript Accepted: February 16, 2012
Published: March 30, 2012

Virtual Issues
Vol. 7, Iss. 6 Virtual Journal for Biomedical Optics

Citation
Emilio Sánchez-Ortiga, Colin J. R. Sheppard, Genaro Saavedra, Manuel Martínez-Corral, Ana Doblas, and Arnau Calatayud, "Subtractive imaging in confocal scanning microscopy using a CCD camera as a detector," Opt. Lett. 37, 1280-1282 (2012)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=ol-37-7-1280

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