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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 7, Iss. 6 — May. 25, 2012

Parallel Raman microspectroscopy using programmable multipoint illumination

Ji Qi and Wei-Chuan Shih  »View Author Affiliations


Optics Letters, Vol. 37, Issue 8, pp. 1289-1291 (2012)
http://dx.doi.org/10.1364/OL.37.001289


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Abstract

We present a novel parallel Raman microspectroscopy scheme for simultaneously collecting Raman spectra from multiple points. This scheme is realized by projectinga multiple-point laser illumination pattern using a spatial light modulator (SLM) and wide-field Raman imaging collection. We demonstrate the performance of this scheme using uniform samples, trapped polymer microparticles and fixed polymer microparticles with mixed molecular composition within a 50×50μm2 field of view. This scheme enables the acquisition of Raman spectra from as many as 40 points simultaneously using a single illumination pattern and detector recording frame without scanning.

© 2012 Optical Society of America

OCIS Codes
(300.6230) Spectroscopy : Spectroscopy, coherent anti-Stokes Raman scattering
(110.4234) Imaging systems : Multispectral and hyperspectral imaging
(350.4855) Other areas of optics : Optical tweezers or optical manipulation

ToC Category:
Imaging Systems

History
Original Manuscript: February 22, 2012
Revised Manuscript: February 28, 2012
Manuscript Accepted: February 28, 2012
Published: April 4, 2012

Virtual Issues
Vol. 7, Iss. 6 Virtual Journal for Biomedical Optics

Citation
Ji Qi and Wei-Chuan Shih, "Parallel Raman microspectroscopy using programmable multipoint illumination," Opt. Lett. 37, 1289-1291 (2012)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=ol-37-8-1289

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Supplementary Material


» Media 1: AVI (142 KB)     
» Media 2: AVI (2716 KB)     

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