We present a fast and flexible fluorescence lifetime imaging microscopy which uses a two-dimensional acousto-optic deflector to achieve fast beam scanning across the sample and provides random access to the regions of interests (ROI). Experimental results using standard fluorescent dye and biological samples show that this system can make addressable fluorescence lifetime measurements and perform fast and flexible fluorescence lifetime imaging particularly to the discontinuous ROI in the sample.
© 2013 Optical Society of America
Original Manuscript: February 11, 2013
Revised Manuscript: April 5, 2013
Manuscript Accepted: April 5, 2013
Published: May 13, 2013
Vol. 8, Iss. 6 Virtual Journal for Biomedical Optics
Jing Qi, Yonghong Shao, Lixin Liu, Kaige Wang, Tongsheng Chen, Junle Qu, and Hanben Niu, "Fast flexible multiphoton fluorescence lifetime imaging using acousto-optic deflector," Opt. Lett. 38, 1697-1699 (2013)