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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics


  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 8, Iss. 7 — Aug. 1, 2013

Fiber-optic two-photon optogenetic stimulation

K. Dhakal, L. Gu, B. Black, and S. K. Mohanty  »View Author Affiliations

Optics Letters, Vol. 38, Issue 11, pp. 1927-1929 (2013)

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Optogenetic stimulation of genetically targeted cells is proving to be a powerful tool in the study of cellular systems, both in vitro and in vivo. However, most opsins are activated in the visible spectrum, where significant absorption and scattering of stimulating light occurs, leading to low penetration depth and less precise stimulation. Since we first (to the best of our knowledge) demonstrated two-photon optogenetic stimulation (TPOS), it has gained considerable interest in the probing of cellular circuitry by precise spatial modulation. However, all existing methods use microscope objectives and complex scanning beam geometries. Here, we report a nonscanning method based on multimode fiber to accomplish fiber-optic TPOS of cells.

© 2013 Optical Society of America

OCIS Codes
(120.3890) Instrumentation, measurement, and metrology : Medical optics instrumentation
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology
(170.0180) Medical optics and biotechnology : Microscopy

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: April 15, 2013
Revised Manuscript: April 28, 2013
Manuscript Accepted: May 6, 2013
Published: May 28, 2013

Virtual Issues
Vol. 8, Iss. 7 Virtual Journal for Biomedical Optics
July 9, 2013 Spotlight on Optics

K. Dhakal, L. Gu, B. Black, and S. K. Mohanty, "Fiber-optic two-photon optogenetic stimulation," Opt. Lett. 38, 1927-1929 (2013)

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