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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 8, Iss. 8 — Sep. 4, 2013

Correction of cell-induced optical aberrations in a fluorescence fluctuation microscope

Charles-Edouard Leroux, Alexei Grichine, Irène Wang, and Antoine Delon  »View Author Affiliations


Optics Letters, Vol. 38, Issue 14, pp. 2401-2403 (2013)
http://dx.doi.org/10.1364/OL.38.002401


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Abstract

We describe the effect of optical aberrations on fluorescence fluctuations microscopy (FFM), when focusing through a single living cell. FFM measurements are performed in an aqueous fluorescent solution and prove to be a highly sensitive tool to assess the optical aberrations introduced by the cell. We demonstrate an adaptive optics (AO) system to remove the aberration-related bias in the FFM measurements. Our data show that AO is not only useful when imaging deep in tissues but also when performing FFM measurements through a single cellular layer. This work paves the way for the application of FFM to complex three-dimensional multicellular samples.

© 2013 Optical Society of America

OCIS Codes
(010.1080) Atmospheric and oceanic optics : Active or adaptive optics
(170.6280) Medical optics and biotechnology : Spectroscopy, fluorescence and luminescence
(180.1790) Microscopy : Confocal microscopy

ToC Category:
Microscopy

History
Original Manuscript: April 24, 2013
Revised Manuscript: June 6, 2013
Manuscript Accepted: June 6, 2013
Published: July 5, 2013

Virtual Issues
Vol. 8, Iss. 8 Virtual Journal for Biomedical Optics

Citation
Charles-Edouard Leroux, Alexei Grichine, Irène Wang, and Antoine Delon, "Correction of cell-induced optical aberrations in a fluorescence fluctuation microscope," Opt. Lett. 38, 2401-2403 (2013)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=ol-38-14-2401


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References

  1. M. A. Digman and E. Gratton, Annu. Rev. Phys. Chem. 62, 645 (2011). [CrossRef]
  2. E. Haustein and P. Schwille, Annu. Rev. Biophys. Biomol. Struct. 36, 151 (2007). [CrossRef]
  3. E. L. Elson, J. Biomed. Opt. 9, 857 (2004). [CrossRef]
  4. J. Enderlein, I. Gregor, D. Patra, and J. Fitter, Curr. Pharm. Biotechnol. 5, 155 (2004). [CrossRef]
  5. S. T. Hess and W. W. W. Webb, Biophys. J. 83, 2300 (2002). [CrossRef]
  6. Rüttinger, V. Buschmann, B. Krämer, R. Erdmann, R. Macdonald, and F. Koberling, J. Microsc. 232, 343 (2008). [CrossRef]
  7. C. Leroux, I. Wang, J. Derouard, and A. Delon, Opt. Express 19, 26839 (2011). [CrossRef]
  8. D. Débarre, E. Botcherby, T. Watanabe, S. Srinivas, M. Booth, and T. Wilson, Opt. Lett. 34, 2495 (2009). [CrossRef]
  9. D. E. Koppel, Phys. Rev. A 10, 1938 (1974). [CrossRef]
  10. M. Booth, M. Neil, and T. Wilson, J. Opt. Soc. Am. A 19, 2112 (2002). [CrossRef]
  11. M. A. Digman, C. M. Brown, P. Sengupta, P. W. Wiseman, A. R. Horwitz, and E. Gratton, Biophys. J. 89, 1317 (2005). [CrossRef]
  12. M. A. Digman, P. Sengupta, P. W. Wiseman, C. M. Brown, A. R. Horwitz, and E. Gratton, Biophys. J. 88, L33 (2005). [CrossRef]
  13. J. Zeng, P. Mahou, M. Schanne-Klein, E. Beaurepaire, and D. Débarre, Biomed. Opt. Express 3, 1898 (2012). [CrossRef]

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