We have developed a novel noncontact setup to implement a cone shell illumination and detection configuration using axicon lenses for depth-sensitive fluorescence measurements. The setup was demonstrated experimentally to be capable of detecting fluorescence from a two-layered turbid agar phantom with a larger sensitivity to the deep layer and a larger range of sensitivity to either layer than a conventional cone configuration implemented by a microscope objective lens. Furthermore, the axicon lens-based setup eliminates the need of moving the objective lens up or down to achieve depth-sensitive measurements, which effectively improves the consistency of optical coupling and thus would be preferred in a clinical setting.
© 2013 Optical Society of America
Original Manuscript: April 11, 2013
Revised Manuscript: May 27, 2013
Manuscript Accepted: June 6, 2013
Published: July 19, 2013
Vol. 8, Iss. 9 Virtual Journal for Biomedical Optics
Yi Hong Ong and Quan Liu, "Axicon lens-based cone shell configuration for depth-sensitive fluorescence measurements in turbid media," Opt. Lett. 38, 2647-2649 (2013)