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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics


  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 8, Iss. 9 — Oct. 2, 2013

Motility-, autocorrelation-, and polarization-sensitive optical coherence tomography discriminates cells and gold nanorods within 3D tissue cultures

Amy L. Oldenburg, Raghav K. Chhetri, Jason M. Cooper, Wei-Chen Wu, Melissa A. Troester, and Joseph B. Tracy  »View Author Affiliations

Optics Letters, Vol. 38, Issue 15, pp. 2923-2926 (2013)

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We propose a method for differentiating classes of light scatterers based upon their temporal and polarization properties computed from time series of polarization-sensitive optical coherence tomography (PS-OCT) images. The amplitude (motility) and time scale (autocorrelation decay time) of the speckle fluctuations are combined with the cross-polarization pixel-wise to render Motility-, autocorrelation-, and polarization-sensitive (MAPS) OCT contrast images. This combination of metrics provides high specificity for discriminating diffusive gold nanorods and mammary epithelial cell spheroids within 3D tissue culture, based on their unique MAPS signature. This has implications toward highly specific contrast in molecular (nanoparticle-based) and functional (cellular activity) imaging using standard PS-OCT hardware.

© 2013 Optical Society of America

OCIS Codes
(030.6140) Coherence and statistical optics : Speckle
(110.2960) Imaging systems : Image analysis
(110.4500) Imaging systems : Optical coherence tomography
(290.5855) Scattering : Scattering, polarization
(110.6915) Imaging systems : Time imaging

ToC Category:
Imaging Systems

Original Manuscript: June 7, 2013
Manuscript Accepted: July 9, 2013
Published: August 1, 2013

Virtual Issues
Vol. 8, Iss. 9 Virtual Journal for Biomedical Optics

Amy L. Oldenburg, Raghav K. Chhetri, Jason M. Cooper, Wei-Chen Wu, Melissa A. Troester, and Joseph B. Tracy, "Motility-, autocorrelation-, and polarization-sensitive optical coherence tomography discriminates cells and gold nanorods within 3D tissue cultures," Opt. Lett. 38, 2923-2926 (2013)

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  1. F. E. Robles, C. Wilson, G. Grant, and A. Wax, Nat. Photonics 5, 744 (2011). [CrossRef]
  2. A. L. Oldenburg, G. Wu, D. Spivak, F. Tsui, A. S. Wolberg, and T. H. Fischer, IEEE J. Sel. Top. Quantum Electron. 18, 1100 (2012). [CrossRef]
  3. D. C. Adler, S.-W. Huang, R. Huber, and J. G. Fujimoto, Opt. Express 16, 4376 (2008). [CrossRef]
  4. M. C. Skala, M. J. Crow, A. Wax, and J. A. Izatt, Nano Lett. 8, 3461 (2008). [CrossRef]
  5. D. Jacob, R. L. Shelton, and B. E. Applegate, Opt. Express 18, 12399 (2010). [CrossRef]
  6. J. F. de Boer, T. E. Milner, M. J. van Gemert, and J. S. Nelson, Opt. Lett. 22, 934 (1997). [CrossRef]
  7. J. Kalkman, R. Sprik, and T. G. van Leeuwen, Phys. Rev. Lett. 105, 198302 (2010). [CrossRef]
  8. V. J. Srinivasan, H. Radhakrishnan, E. H. Lo, E. T. Mandeville, J. Y. Jiang, S. Barry, and A. E. Cable, Biomed. Opt. Express 3, 612 (2012). [CrossRef]
  9. J. Lee, H. Radhakrishnan, W. Wu, A. Daneshmand, M. Climov, C. Ayata, and D. A. Boas, J. Cereb. Blood Flow Metab. 33, 819 (2013). [CrossRef]
  10. G. Farhat, A. Mariampillai, V. X. D. Yang, G. J. Czarnota, and M. C. Kolios, J. Biomed. Opt. 16, 070505 (2011). [CrossRef]
  11. A. L. Oldenburg, R. K. Chhetri, D. B. Hill, and B. Button, Biomed. Opt. Express 3, 1978 (2012). [CrossRef]
  12. M. J. Bissell and D. Radisky, Nat. Rev. Cancer 1, 46 (2001). [CrossRef]
  13. R. K. Chhetri, Z. F. Phillips, M. A. Troester, and A. L. Oldenburg, PLoS One 7, e49148 (2012). [CrossRef]
  14. A. L. Oldenburg, M. N. Hansen, T. S. Ralston, A. Wei, and S. A. Boppart, J. Mater. Chem. 19, 6407 (2009). [CrossRef]
  15. J. M. Tucker-Schwartz, T. A. Meyer, C. A. Patil, C. L. Duvall, and M. C. Skala, Biomed. Opt. Express 3, 2881 (2012). [CrossRef]
  16. Y. Jung, R. Reif, Y. Zeng, and R. K. Wang, Nano Lett. 11, 2938 (2011). [CrossRef]
  17. A. M. Alkilany, L. B. Thompson, S. P. Boulos, P. N. Sisco, and C. J. Murphy, Adv. Drug Deliv. Rev. 64, 190 (2012). [CrossRef]
  18. R. K. Chhetri, K. A. Kozek, A. C. Johnston-Peck, J. B. Tracy, and A. L. Oldenburg, Phys. Rev. E 83, 040903 (2011). [CrossRef]
  19. A. L. Oldenburg and R. K. Chhetri, Proc. SPIE 7889, 78891V (2011).
  20. B. Nikoobakht and M. A. El-Sayed, Chem. Mater. 15, 1957 (2003). [CrossRef]
  21. C. F. Bohren and D. R. Huffman, Absorption and Scattering of Light by Small Particles (Wiley, 1983).
  22. N. Khlebtsov, A. Mel’nikov, V. Bogatyrev, A. Alekseeva, and B. Khlebtsov, Opt. Spectrosc. 100, 448 (2006). [CrossRef]
  23. P. Casbas-Hernandez, M. D’Arcy, E. Roman-Perez, H. A. Brauer, K. McNaughton, R. K. Chhetri, A. L. Oldenburg, and M. A. Troester, Breast Cancer Res. (2013) (to be published).
  24. D. D. Nolte, R. An, J. Turek, and K. Jeong, Biomed. Opt. Express 3, 2825 (2012). [CrossRef]
  25. J. Lee, W. Wu, J. Y. Jiang, B. Zhu, and D. A. Boas, Opt. Express 20, 22262 (2012). [CrossRef]
  26. S. Prabhulkar, J. Matthews, S. Rawal, and R. M. Awdeh, Investig. Ophthalmol. Vis. Sci. 54, 1192 (2013). [CrossRef]

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