Recently developed spatial light modulator (SLM)-based differential interference contrast (DIC) microscopy [Opt. Lett. 34, 2988 (2009)] reveals flexibility on the implementation of DIC imaging. However, its numerical aperture (spatial resolution) is limited to maintain sufficient interference contrast, because it requires two beams to interfere. We present a structured illumination (SI) SLM-based DIC microscopy to effectively improve the lateral resolution of the SLM-based DIC microscopy. The SI field is generated and controlled by an adjustable grating displayed on an SLM. The SI SLM-based DIC expands the bandwidth of the coherent transfer function of the SLM-based DIC imaging system, thus improving the spatial resolution. The reconstructed SI SLM-based DIC image exhibits lateral resolution of approximately 208 nm, doubling that of the common SLM-based DIC image (approximately 415 nm). SI SLM-based DIC microscopy has the potential for achieving high-resolution quantitative phase images.
© 2013 Optical Society of America
Original Manuscript: July 5, 2013
Manuscript Accepted: July 22, 2013
Published: August 19, 2013
Vol. 8, Iss. 10 Virtual Journal for Biomedical Optics
Jianling Chen, Xiaohua Lv, and Shaoqun Zeng, "Doubling the resolution of spatial-light-modulator-based differential interference contrast microscopy by structured illumination," Opt. Lett. 38, 3219-3222 (2013)