OSA's Digital Library

Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics


  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 8, Iss. 10 — Nov. 8, 2013

Experimental and theoretical analysis for improved microscope design of optical projection tomographic microscopy

Ryan L. Coe and Eric J. Seibel  »View Author Affiliations

Optics Letters, Vol. 38, Issue 17, pp. 3398-3401 (2013)

View Full Text Article

Enhanced HTML    Acrobat PDF (353 KB)

Browse Journals / Lookup Meetings

Browse by Journal and Year


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools



We present theoretical and experimental results of axial displacement of objects relative to a fixed condenser focal plane (FP) in optical projection tomographic microscopy (OPTM). OPTM produces three-dimensional, reconstructed images of single cells from two-dimensional projections. The cell rotates in a microcapillary to acquire projections from different perspectives where the objective FP is scanned through the cell while the condenser FP remains fixed at the center of the microcapillary. This work uses a combination of experimental and theoretical methods to improve the OPTM instrument design.

© 2013 Optical Society of America

OCIS Codes
(110.2990) Imaging systems : Image formation theory
(180.0180) Microscopy : Microscopy
(050.1755) Diffraction and gratings : Computational electromagnetic methods

ToC Category:
Imaging Systems

Original Manuscript: May 8, 2013
Revised Manuscript: August 5, 2013
Manuscript Accepted: August 5, 2013
Published: August 26, 2013

Virtual Issues
Vol. 8, Iss. 10 Virtual Journal for Biomedical Optics

Ryan L. Coe and Eric J. Seibel, "Experimental and theoretical analysis for improved microscope design of optical projection tomographic microscopy," Opt. Lett. 38, 3398-3401 (2013)

Sort:  Author  |  Year  |  Journal  |  Reset  


  1. Q. Miao, A. Reeves, F. Patten, and E. Seibel, Ann. Biomed. Eng. 40, 263 (2012). [CrossRef]
  2. M. Meyer, M. Fauver, J. Rahn, T. Neumann, and F. Patten, Pattern Recogn. 42, 141 (2009). [CrossRef]
  3. M. Meyer, F. Patten, T. Neumann, J. Hayenga, D. Steinhauer, J. Rahn, and A. Nelson, J. Thorac. Oncol. 4, S378 (2009).
  4. M. Fauver, E. Seibel, J. Rahn, M. Meyer, F. Patten, T. Neumann, and A. Nelson, Opt. Express 13, 4210 (2005). [CrossRef]
  5. R. Coe and E. Seibel, J. Opt. Soc. Am. A 29, 2696 (2012). [CrossRef]
  6. R. L. Coe and E. J. Seibel, Proc. SPIE 8592, 85920G (2013). [CrossRef]
  7. I. Çapoglu, C. White, J. Rogers, H. Subramanian, A. Taflove, and V. Backman, Opt. Lett. 36, 1596 (2011). [CrossRef]
  8. A. C. Kak and M. Slaney, Principles of Computerized Tomographic Imaging (IEEE, 1988).
  9. E. Botcherby, R. Juškaitis, M. Booth, and T. Wilson, Opt. Lett. 32, 2007 (2007). [CrossRef]
  10. C. D. Kuglin and D. C. Hines, in Proceedings of IEEE International Conference on Systems, Man and Cybernetics (IEEE, 1975), p. 163.
  11. K. F. Chou, Q. Miao, R. L. Coe, and E. J. Seibel, J. Cytol. Histol. S2, 001 (2012). [CrossRef]

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited