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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics


  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 8, Iss. 4 — May. 22, 2013

Single-image far-field subdiffraction limit imaging with axicon

Craig Snoeyink and Steve Wereley  »View Author Affiliations

Optics Letters, Vol. 38, Issue 5, pp. 625-627 (2013)

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This Letter presents a technique for subdiffraction limit imaging termed Bessel beam microscopy (BBM). By placing a lens in series with an axicon in the optical path of a microscope, the diffraction-limited resolution of the base microscope is improved by one third. This improvement is demonstrated experimentally by resolving individual subdiffraction limit fluorescent beads in a close-pack arrangement. The behavior of the BBM system is explored using angular diffraction simulations, demonstrating the possibility of resolving features spaced as little as 110 nm apart when viewed with a 100×1.4NA objective. Unique among super-resolution techniques, BBM acquires subdiffraction limit information in a single image with broadband unstructured illumination using only static geometric optics placed between the microscope and camera.

© 2013 Optical Society of America

OCIS Codes
(110.0180) Imaging systems : Microscopy
(180.2520) Microscopy : Fluorescence microscopy

ToC Category:
Imaging Systems

Original Manuscript: November 5, 2012
Revised Manuscript: January 3, 2013
Manuscript Accepted: January 11, 2013
Published: February 21, 2013

Virtual Issues
Vol. 8, Iss. 4 Virtual Journal for Biomedical Optics

Craig Snoeyink and Steve Wereley, "Single-image far-field subdiffraction limit imaging with axicon," Opt. Lett. 38, 625-627 (2013)

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