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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 8, Iss. 4 — May. 22, 2013

Axial potential mapping of optical tweezers for biopolymer stretching: the bead size matters

Arash Ahmadi and S. Nader S. Reihani  »View Author Affiliations


Optics Letters, Vol. 38, Issue 5, pp. 685-687 (2013)
http://dx.doi.org/10.1364/OL.38.000685


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Abstract

Optical tweezers (OT) are widely used for pico (and femto)-Newton range force measurements. The appropriate choice of the bead size is not well understood for biopolymer stretching applications of OT. We have shown, both by theory and experiment, that wrong choice of the bead size could cause errors as large as 295% in the measured force. We provide a simple map for correct choice of the bead size and the direction of pulling for such applications. There is a good agreement between our theoretical and experimental results.

© 2013 Optical Society of America

OCIS Codes
(140.7010) Lasers and laser optics : Laser trapping
(170.4520) Medical optics and biotechnology : Optical confinement and manipulation
(350.4855) Other areas of optics : Optical tweezers or optical manipulation

ToC Category:
Optical Tweezers or Optical Manipulation

History
Original Manuscript: November 12, 2012
Revised Manuscript: January 1, 2013
Manuscript Accepted: January 1, 2013
Published: February 25, 2013

Virtual Issues
Vol. 8, Iss. 4 Virtual Journal for Biomedical Optics

Citation
Arash Ahmadi and S. Nader S. Reihani, "Axial potential mapping of optical tweezers for biopolymer stretching: the bead size matters," Opt. Lett. 38, 685-687 (2013)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=ol-38-5-685

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