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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 8, Iss. 4 — May. 22, 2013

Surface-plasmon-coupled emission microscopy with a polarization converter

Yikai Chen, Douguo Zhang, Lu Han, Guanghao Rui, Xiangxian Wang, Pei Wang, and Hai Ming  »View Author Affiliations


Optics Letters, Vol. 38, Issue 5, pp. 736-738 (2013)
http://dx.doi.org/10.1364/OL.38.000736


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Abstract

Although surface-plasmon-coupled emission-based fluorescence microscopy proves high sensitivity for surface imaging, its donut shape point spread function (PSF) leads to low optical resolution and inefficient signal collection. In this Letter, we experimentally demonstrate the feasibility of solving this problem by the use of a liquid-crystal plate, which could convert the polarization state of surface-plasmon-coupled fluorescence from radial to linear. After being focused by the collection lens, an Airy disk-like PSF of small size can be realized. Experimental results reveal that both the lateral resolution and the signal-to-noise ratio can be enhanced simultaneously.

© 2013 Optical Society of America

OCIS Codes
(180.2520) Microscopy : Fluorescence microscopy
(240.6680) Optics at surfaces : Surface plasmons
(260.5430) Physical optics : Polarization

ToC Category:
Optics at Surfaces

History
Original Manuscript: November 26, 2012
Manuscript Accepted: January 27, 2013
Published: February 26, 2013

Virtual Issues
Vol. 8, Iss. 4 Virtual Journal for Biomedical Optics

Citation
Yikai Chen, Douguo Zhang, Lu Han, Guanghao Rui, Xiangxian Wang, Pei Wang, and Hai Ming, "Surface-plasmon-coupled emission microscopy with a polarization converter," Opt. Lett. 38, 736-738 (2013)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=ol-38-5-736


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