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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 8, Iss. 4 — May. 22, 2013

Simultaneous angular multiplexing optical projection tomography at shifted focal planes

Lingling Chen, Natalie Andrews, Sunil Kumar, Paul Frankel, James McGinty, and Paul M. W. French  »View Author Affiliations


Optics Letters, Vol. 38, Issue 6, pp. 851-853 (2013)
http://dx.doi.org/10.1364/OL.38.000851


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Abstract

We describe an angular multiplexing technique for optical projection tomography that improves resolution, signal-to-noise ratio, and imaging speed by ameliorating the trade-off between spatial resolution and depth of field and improving the light collection efficiency. Here we demonstrate that imaging at two orthogonal angular projections simultaneously, focused on shifted planes in the sample, improves the average spatial resolution by 20% and the light collection efficiency by a factor of 4, thereby enabling increased acquisition speed and reduced light dose.

© 2013 Optical Society of America

OCIS Codes
(170.6900) Medical optics and biotechnology : Three-dimensional microscopy
(170.6960) Medical optics and biotechnology : Tomography

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: January 2, 2013
Manuscript Accepted: January 29, 2013
Published: March 11, 2013

Virtual Issues
Vol. 8, Iss. 4 Virtual Journal for Biomedical Optics

Citation
Lingling Chen, Natalie Andrews, Sunil Kumar, Paul Frankel, James McGinty, and Paul M. W. French, "Simultaneous angular multiplexing optical projection tomography at shifted focal planes," Opt. Lett. 38, 851-853 (2013)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=ol-38-6-851


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