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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics


  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 9, Iss. 3 — Mar. 6, 2014

Quantitative cerebral blood flow imaging with extended-focus optical coherence microscopy

Arno Bouwens, Tristan Bolmont, Daniel Szlag, Corinne Berclaz, and Theo Lasser  »View Author Affiliations

Optics Letters, Vol. 39, Issue 1, pp. 37-40 (2014)

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Quantitative three-dimensional blood flow imaging is a valuable technique to investigate the physiology of the brain. Two-photon microscopy (2PM) allows quantification of the local blood flow velocity with micrometric resolution by performing repeated line scans, but prohibitively long measurement times would be required to apply this technique to full three-dimensional volumes. By multiplexing the image acquisition over depth, Fourier domain optical coherence tomography (FDOCT) enables quantification of blood flow velocities with a high volume acquisition rate, albeit at a relatively low spatial resolution. Extended-focus optical coherence microscopy (xfOCM) increases the lateral resolution without sacrificing depth of field and therefore combines the high volume acquisition rate of FDOCT with a resolution comparable to 2PM. Here, we demonstrate high-resolution quantitative imaging of the blood flow velocity vector’s magnitude in the adult murine brain with xfOCM.

© 2013 Optical Society of America

OCIS Codes
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.4500) Medical optics and biotechnology : Optical coherence tomography
(170.5380) Medical optics and biotechnology : Physiology
(170.6900) Medical optics and biotechnology : Three-dimensional microscopy

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: September 23, 2013
Revised Manuscript: October 31, 2013
Manuscript Accepted: November 15, 2013
Published: December 19, 2013

Virtual Issues
Vol. 9, Iss. 3 Virtual Journal for Biomedical Optics

Arno Bouwens, Tristan Bolmont, Daniel Szlag, Corinne Berclaz, and Theo Lasser, "Quantitative cerebral blood flow imaging with extended-focus optical coherence microscopy," Opt. Lett. 39, 37-40 (2014)

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